AND SPECIES HYBRIDS WITHIN THE GENUS SACCHARUM 135 
so regularly arranged as in the glagah-plates. This makes the count 
more difficult. The difficulty is to distinguish between gemini and chro- 
mosomes already in the first-stage of anaphase. Of the latter, one sees, 
of course two, lying one above the other, when observed from one of 
the poles, while focussing at two different levels. As of such a pair, the 
one chromosome is sometimes shifted a little in regard to the other, it 
may happen that one such a geminus is counted for two. Fig. 29 pic- 
tures a nuclear plate which doubtless contains 40 chromosomes, all 
visible at the same adjustment of the microscope, although some a 
little cloudy. 

Fig. 29. Fig. 30. 
Fig. 29. Metaphase of the heterotype division of Ardjoeno (regular division) 
viewed form one of the poles x 2300. 
Fig. 30. Anaphase of the heterotype division of Ardjoeno (regular division) vie- 
wed from the side x 2300. 
In this case also some, particularly the smallest ones, were already 
in the first stage of anaphase. As during anaphase the one chromosome 
always covered the other, the upper one alone could be drawn. 
In 21 counts of metaphase the number found was 40; once I hesita- 
ted between 39 and 40, twice between 40 and 41. 
Fig. 30 pictures an anaphase viewed from the side; in as much as 
some chromosomes covered the others, not all could be drawn. One 
