144 A CYTOLOGICAL INVESTIGATION OF SOME SPECIES 
perpendicular to the optical field. If one counts each time two ana- 
phase-chromosomes as one, a total of 46 is obtained, which points to- 
wards 12 unpaired chromosomes, a number fitting in well with the 
numbers which can appear in anaphase. 
Twenty one counts of nuclear plates of G. G. N. G. gave 4 times hesi- 
tation between 40 and 42; three times 41; seven times: 42; twice 43; 
thrice 44; once 45; and once 46. 
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Fig. 44 

Fig. 45. 
Fig. 44. Anaphase of the heterotype division of G. G. N. G. view ed from one of 
the poles (irregular division) x 2300. 
Fig. 45. PMC of G.G. N.G.in telophase x 2300. 
Anaphase counts can be made only when the distance between the 
chromosomes moving towards the poles and those lagging behind in the 
aequator is sufficiently great, to distinguish the latter clearly from the 
former. Also the chromosomes of the groups moving apart may not 
have approached too closely to the poles, because in that case they are 
lying too close together. Fig. 44 pictures an anaphase where each of the 
groups moving apart contains without any doubt 38 chromosomes, 
while 4 unpaired ones have lagged behind on the aequator. The latter 
ones are tinted grey in the figure and drawn next to the chromosomes 
of the group which was visible when adjusting the microscope at a high 
level. It should be noticed that the unpaired chromosomes all are lying 
at the border of the aequator. This is not by chance; one can always 
