bn 
AND SPECIES-HYBRIDS OF THE GENUS SACCHARUM 275 
mosomes are seen in the drawing lying close together at the upper-end 
of the nucleus, adpressed to its wall. Nearly always a larger number of 
unpaired chromosomes is present, as in fig. 56, where 1 set of unpaired 
chromosomes is seen at the left and 4 sets at the right side of the nucleus 
so that 35 gemini and 10 unpaired chromosomes are present. By coun- 
ting always 2 unpaired chromosomes as | geminus, the results of 16 
counts varied from 38 to 42; the preparations however were frequently 
very indistinct on account of the frequent presence of small nucleoli. 

Fig. 55. 
Fig. 55. Pollen-mothercell of Bandjermasin hitam in diakinesis x 2300. 
Counts of nuclearplates of Bandjermasin hitam were impossible as the 
chromosomes were too much crowded. During anaphase unpaired chro- 
mosomes are always present in variable number. In fig. 57 a spindle is 
seen with 4 unpaired chromosomes, all of which however have already 
been split longitudinally. In the frequent occurrence of unpaired chro- 
mosomes Bandjermasin hitam agrees fully with G. D. N. G. 
A preliminary investigation of some other kinds of sugarcane also 
gave 40 as the probable haploid chromosome number. Fig. 58 shows a 
nuclear plate of Teboe Sampang A, in which all of the 40 chromosomes 
were lying in one level. 
