AND SPECIES-HYBRIDS OF THE GENUS SACCHARUM 279 
total number of chromosomes can not be stated with absolute certain- 
ty, but approximates 80 closely. 
At the end of diakinesis the chromosomes are much shortened and 
almost as broad as long, so that it can no longer be observed that they 
belong two by two together. 

Fig. 63. 

Fig. 62. 
Fig. 62. PMC of G. G. N. G. in diakinesis (abnormal division) x 2300. 
Fig. 63. Metaphase of an abnormal division of a PMC of G. G. N. G. x 2300. 
In the nuclear plate the chromosomes lie in approximately diploid 
number. This can be seen in fig. 63. The chromosomes, which here are 
univalent, are clearly smaller than gemini and possess an other shape; 
some show indications of longitudinal splitting. Most chromosomes 
lie exactly in the aequatorial plane, a few however become visible when 
focussing at a higher or lower level. Their number is approximately 80. 
Seen from the side, one never sees the chromosomes all in one plane. 
Nowhere nuclear spindles are visible. This is perhaps due to the great 
transparency of the cytoplasm but it may also be due to a non-deve- 
lopment or to but a slight development of the spindle. 
During anaphase one never sees the chromosomes reach the poles ; 
