292 A CYTOLOGICAL INVESTIGATION OF SOME SPECIES 
In 11 metaphase-counts, numbers from 46 to 48 were found. Fig. 84 
pictures a nuclear plate with approximately 48 chromosomes. 
Anaphase counts of Ruckree II could not be made. 
Doubtless the chromosomenumber of Ruckree is about the same as 
that of Chunnee; the possibility of a small difference between the two 
is however not excluded, but it would be a very laborious task to settle 
this point, as one is limited to anaphase-counts, while only 3 anaphases, 
in which the chromosomes could be counted, were met with in a large 
number of Chunnee-preparations. 
Figures 85, 86 and 87 picture a prophase, a metaphase and an ana- 
phase of dividing, somatic, Chunnee-cells. As the chromosomes are very 
small, elongated and curved, an entirely exact count was not possible; 
nor was it in the case of Ruckree. In the drawings the chromosomes are . 
drawn slightly more apart, than in the observed microscopical images. 
In reality the chromosomes occasionally lie so exactly in each other’s 
prolongation that their number cannot be determined with absolute 
certainty. The presence of slightly stained protoplasmic unions be- 
tween the chromosomes causes an additional difficulty. Doubtless ho- 
wever, the chromosome number of the somatic nuclei of Chunnee and 
Ruckree does not differ much from 90. 
He GHAPTERAELI 
DISCUSSION OF THE RESULTS OF THE FORMER CHAPTER IN CONNEXION 
WITH THE LITERATURE 
It results from the investigation, that the haploid chromosome- 
number of Saccharum officinarum is 40, while that of S. spontaneum is 
56. In the case of the British Indian canes Chunnee and Ruckree IT, the 
number of chromatine-elements in diakinesis and metaphase of the 
heterotype division turned out tot be 45—50, while the differences 
were due to the presence of a smaller or larger number of univalent 
chromosomes. It could be derived from diaster-stages of PMC that the 
diploid chromosome number of Chunnee should be 90 or 91. The „chro- 
mosome-number which previous investigations FRANCK !) and Kuwa- 
1) W. J. FRANCK, Somatische kern- en celdeeling en microsporogenese bij het 
suikerriet. Dissertatie 1911. 
