
THE CYTOLOGY OF THE SUGARCANE 505 
chromosomes into which they will split, have already begun to separ- 
ate. In the metaphase of 
Koesoemo here pictured 
however, the chromosomes 
are not lying in one plane; 
one meets with smaller, 
more or less round, unival- 
ent, chromosomes, besides 
larger diamond shaped or 
elongated, laced, bivalent 
ones. Some univalent chro- 
mosomes are arranged in 

pairs, they are derived 
from gemini and have pas- 
iGo: 
Aaron das h _ Metaphase of the heterotype division of Koe- 
sed already into anaphase; soemo viewed from the side. X 2300. 
in the case of most ofthem 
this has not occurred, they have reached, as univalent chromosomes, 
the aequator or are near to it. One chromosome is situated, at a consi- 
derable distance from the aequator on the spindle; such a one could 
be observed on many spindles. 
The occurrence of many univalent chromosomes made the counting, 
even in anaphase, difficult. The chromosomes in the groups moving 
towards the poles were situated at very different levels. In anaphase 
90—93 chromosomes were counted. Fig. 9 pictures a rather clear ana- 
phase in which 93 or, possibly, 92 chromosomes occurred. The diploid 
number of Koesoemo consequently is 92 or 93. 
Not only the heterotype division but the homotype one also, shows 
great irregularities in the case of 8 
this cane. The nuclei of the dyads 4° 3 „ ® gee 
SR: 8 @ e® © 
already, are of a deviating type. It g el = > I ® à ix tee 
is the rule among various kinds of B#@ x a e a gen? 
we 4% ps 
sugarcane that the chromosomes “ge 0% 
remain more or less distinctly vis- ee® © 
-ible in the dyad-nuclei, while in the Fıc. 9. 
case of Koesomo but a few threads Anaphase of the heterotype division of 
Koesoemo, viewed from the side. x 2300. 
remain visible and numerous large 
and smaller nucleoli appear. In the metaphase of the homotype div- 
ision the chromosomes were not arranged exactly in one plane. Both 
