506 THE CYTOLOGY OF THE SUGARCANE 
unusually large chromosomes, sometimes very similar to nucleoli, 
and unusually small chromosomes are present. 
Also during the anaphase of this division the difference in size bet- 
ween the chromosomes is very conspicuous. It looks as if a kind of de- 
generation of the chromosomes sets in. Moreover the anaphase shows 
that many chromosomes lag behind, such ones being frequently 
stretched so as to form long strings. It is rather common for large nu- 
cleolus-like bodies to appear on the spindle during telophase. In the 
tetrad-nuclei also often several very large nucleoli are present while 
such are met with occasionally also in the cyto plasm of the tetrad- 
cells. | 
EK 2 (Derived probably from Lahaina x Fidji) Both entirely re- 
gular and irregular divisions of the pollenmothercells were observed in 
the case of this cane. In several diakinesisnuclei 40 chromosomes, pro- 
bably all bivalent ones, were seen. 
Fig. 10 shows a nucleus in which 2 
univalent chromosomes occur, the 39 
other ones being probably bivalent. The 
two univalent. ones are seen next to one 
another on the left side of the figure. In 
the metaphase sometimes 40, sometimes 
more than 40 chromosome&s, were seen, 
which must be due to the presence of 
univalent ones. But one anaphase in which 
the chromosomes could be counted occ- 
Fir. 10. urred amongst the preparations, it con- 
Nucleus of a pollenmothercell tained 80 chromosomes. The haploid 
of EK rin diakinesis. x 2300. 

chromosomenumber of EK 2 is therefore 
doubtless 40, the diploid one 80. 
EK 28 (Perhaps derived from 100 PO] x EK 2) Preparations were 
made from flowers of six different inflorescences, all from the garden of 
the experiment-station: one inflorescence had been gathered in 1920, 
all the other ones in 1923. The division of the pape ee was al- 
ways irregular; regular division did not occur. 
Fig. 11 pictures a diakinesis-nucleus with, in all probability, 38 bi- 
valent and 4 univalent chromosomes. Two of these univalent chromo- 
somes are seen at the left of the figure, they are drawn a dark gray and 
are seen close together a little below the middle; one is situated at 


