512 THE CYTOLOGY OF THE SUGARCANE 
Finally „tetrads’’ in which three cells only had been formed occur- 
red, of which only two cells contained a nucleus. 

Fic. 18. Normal and abnormal pollen-tetrads of EK 28. x 1400. 
As a consequence of the setting out of the pairing of a large number 
of chromosomes in the prophase of the division of the pollenmother- 
cells, as occasionally occurs in thecase of EK 28, there results therefore 
a very incomplete chromosome-reduction, moreover the cell-division 
itself can become so abnormal that no normal pollen is formed. 
DISCUSSION OF THE RESULTS OF THIS INVESTIGATION 
It was shown that the original sugarcane kinds Black Cheribon and 
Lahaina possessed 40 chromosomes in the haploid and 80 in the diploid 
phase, just as the other typical representatives of the species Saccha- 
rum officinaram, such as Ardjoeno, Green German New Guinea, Fidji, 
Batjan and Bandjarmassin hitam. 
Loethers-cane has a diploid number of 99 and consequently deviates 
considerably in chromosome-number from Saccharum officinarum. 
JESWIET also considers Loethers to differ in habit from S. officinarum. 
Selffertilised Loethers gave plantlets which are very small, have very 
thin stems, hoary leaves and are very different generally from all Sac- 
charum-species known to us. One of these plantlets 242 B 26 showed 
great deviations in cytology also 1). 
1) 242 B 26 shows very great deviations in the structure of its flowers. In one 
of these flowers for instance 5 anthers and 2 ovaries were found. Both the two 
ovaries and the ovules had an abnormal shape. In one of these ovaries an ovule 
with 2 embryosac mothercells occurred. The anthers in all flowers were abnormal. 
In stead of consisting of 4 cell-layers the wall of the loculi consisted of 5 or 6 layers 
A normal tapetum could not be found. The pollen-mothercells degenerated du- 
ring synapsis, so that no pollen could be formed. 



