514 THE CYTOLOGY OF THE SUGARCANE 
mes in the diploid phase. If the investigation should show that EK 28 
possessed some chromosomes more than 80, such would have been an 
indication that zoo POJ or any other kind with more than 40 chromo- 
somes in the haploid phase had been one of the parents. It turned out 
however that the chromosomenumber of EK 28 was but 80. It was 
therefore of importance to find out how derivatives of 100 POJ be- 
have in this respect; whether they have a chromosome number which 
shows a clear connection with the larger number of the mother. 
or not. 
Unfortunately no seedlings of a cross of 190 POJ with one of the 
forms of Saccharum officinarum were present. I had therefore to resort 
to G 92, an individual, arisen from the cross zoo POJ x Glagahalas 
Troeno (Saccharum spontaneum) while I investigated also 2364 POJ, 
a hybrid of zoo POJ x Kassoer (S. officinarum x spontaneum). 
It was shown already in the first part that hybrids of S. officinarum 
x S. spontaneum have 136 as the diploid chromosome-number. After 
the publication of this first part, I have investigated still several other 
hybrids of this kind, and found that the diploid chromosomenumber 
really is 136. 
In the next publication the results of this investigation will be descri- 
bed. This number 136 could be explained by the assumption that, at the 
time of fertilisation the 40 chromosomes in the eggcell of the sugarcane 
(S. officinarum) have became doubled in number, so that 80 chro- 
mosomes of the sugarcane meet 56 chromosomes of glagah (S. spon- 
taneum). 
Fig. 19 pictures an anaphase of G g2 in which 139 cho were : 
counted; another anaphase of this cane showed the same number. It 
ap She follows that in the case 
© Sebo of 190 POJ also doub- 
x ne eae ling of the chromosomes 
RO 2e has occurred at the fert- 
Es Be ilisationbyamaleglagah 
(S. spontaneum) nucleus. 
EL It follows also that in 

Anaphase of the heterotype division of G 92, vie- the case of G 92 there are 
wed from one of the poles. x 2300. some chromosomes more’ 
present than in the case 
of crosses between S. officinarum and S. spontaneum. As Glagah alas 
