THE CYTOLOGY OF THE SUGARCANE 517 
different years, as the pollen-fertility of certain kinds of sugarcane can 
be very different in different years. 
The investigation of the last years has made the existence of such a 
connection more probable. 
The experimentstation gathered seed in 1920, which had been for- 
med on 3 self-fertilised inflorescences of Ardjoeno, which had grown up 
at Kepandjen near Malang 1). The seed of each of these inflorescences, 
gave a large number of plantlets. Five hundred of these — a compa- 
ratively small part of the total number obtained — were planted out in 
the experiment-garden. 
These Ardjoeno-inflorescences were consequently highly fertile. 
In 1923 one Ardjoeno-inflorescence was selfed in our garden at Pa- 
soeroean ; no seedlings could be obtained from it. 
For the cytological investigation of Ardjoeno, the results of which 
have been described in the first part, an inflorescence was used, 
which also had been gathered in 1920 in Kepandjen. Most of the pollen- 
mothercells in this inflorescence showed a complete reduction-division 
On the contrary two inflorescences of Ardjoeno, gathered in 1923 at 
Pasoeroean, showed almost in all cases an incomplete reduction-divi- 
sion. 
Of 50 anaphases from the preparations of the material of Boemiajoe 
near Kepandjen 27 showed no chromosomes lagging behind. In more 
than one half of the divisions consequently the reduction had been 
complete. In the 23 other anaphases a total of 82 chromosomes lag- 
ging behind was counted. In preparations of the material of Pasoeroean 
40 anaphases could be examined. In one anaphase only a complete re- 
duction-division was found, in the 39 other ones 206 chromosomes lag- 
ged behind. 
EK 28 showed in all inflorescences examined, a very incomplete re- 
duction-division. Pollenmothercells which in the prophase of the divi- 
sion showed no univalent chromosomes, did not occur at all. The uni- 
valent chromosomes were always numerous; in the case of one inflores- 
cence the majority of chromosomes had, even, as a rule, remained univa- 
lent. 
1) This plantation lies at an altitude of approximately 310 M. above sea-level, 
on good soil in a climate with more rain than Passoeroean, where moreover the 
soilis a very heavy clay. 
