Cyclophosphamide [N,N-bis (2-chloroethyl)-N' ,0-propylenediamide ] 
In vivo activation on wheat root meristems occurred only when there 
was pretreatment with caffeine (1115). 
CH 
™~ 
cH” 79 CH,~CH,Cl i} CH,-CH,C1 
| ‘ | N ss + H,N-CH,-cH,-o-p-n’ % % 
CH po ON ae 2 bn Nc. -cH,-c1 
<a aN CH, -CH,C1l rey 
, 0 grey 
Cycocel (CCC) [2-(Chloroethyl) Trimethylammonium Chloride] 
Shoots of barley and chrysanthemums were placed for 24 hours in 
beakers containing labelled CCC. After removal, the shoots and residual 
solutions were tested for radioactivity. Paper chromatography indicated 
the presence of five radioactive products in both barley and chrysanthemum 
shoots. One compound was identified as choline. Additional assays showed 
that three of the compounds were metabolites of choline and indicated that 
the fifth compound was probably an intermediate in the conversion of CCC to 
choline (1276). 
On foliar surfaces, CCC was absorbed slowly and resided as such. 
Rats fed CCC excreted the unchanged compound (125, 216). 
After application of CCC to wheat and barley, CCC was transformed into 
a quaternary base which was transported to the roots. In the leaves of winter 
wheat and spring barley, CCC was metabolized to an unidentified choline 
derivative (1315). 
108 




