Dinitro Compounds (Aromatic) 
DNOC (4,6-Dinitro-o-Cresol32,5-Dinitro-o-cresol) 
{2 ,4-Dinitro-6-hydroxytoluene ] 

In man, DNOC acted as a cumulative poison and was eliminated slowly 
over a period of weeks. Small amounts were eliminated unchaged in the 
; urine (1330). 
When fed to rabbits, five percent was excreted unchanged and 1% as a 
conjugate. The main urinary metabolites were: 6-N-acetylamino-4-nitro-o- 
cresol (1 - 1.52); O-conjugates of this compound (10%). 3-Amino-5-nitro- 
salicylic acid, 4-amino-6-nitro-o-cresol, and derivatives of this cresol 
were excreted in small amounts (1190, 1330, 1423). 
After injection of DNOC in locusts Schistocerca gregaria and Locusta 
migratoria, 6-amino-4-nitro-o-cresol and its acetyl derivative were 
observed. When 6-amino-4-nitro-o-cresol was injected, the corresponding 
glucoside and sulfate were identified as well as the acetyl derivative (788). 
Studies with soil microorganisms showed that aromatic dinitro compounds 
were Slowly degraded (1308, 1663, 1664). When 2,4-dinitrophenol or 2,4- 
dinitro-o-cresol is exposed to bacteria (Pseudomonas) (1664) or an atypical 
Corynebacterium simplex (575) the yellow color bleached with the appearance 
of nitrite. 
A Pseudomona (N.C.1.B. 9771) isolated from garden soil perfused with DNOC 
was capable of metabolizing this herbicide in pure culture. The degradative 
path, indicated by chemical isolation of intermediates, sequential induction, 
and other techniques, was determined (1397). 
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