N-Serve 
[2-Chloro-6-(trichloromethyl) pyridine ] 
cl4_1 abeled N-Serve was fed to a female beagle. Paper chromatography 
of the collected urine was used to isolate metabolites. Mixed melting 
points, UV, paper chromatography, and infrared spectra were used to identify 
the metabolite as N-(6-chloropicolinoyl) glycine (1202, 1203). 
After the ingestion of N-Serve, rats excreted via the urine 6-chloro- 
picolinic acid (mainly) and N-(6-chloropicolinoyl) glycine (1198). 
Studies were conducted with labeled N-Serve and corn, carrots, lettuce, 
tomatoes, wheat and oats. Fractionation and chromatography showed that the 
principal metabolite was 6-chloropicolinic acid (1198, 1199, 1200). Corn 
converted the 6-chloropicolinic acid to 2-chloropyridine. 6-Chloropicolinic 
acid was also conjugated with lipids and protein. Some free Cl was formed 
(970). 
In soil, 2-chloro-6-(trichloromethyl) pyridine was hydrolyzed to 6- 
chloropicolinic acid. Samples of the acid were dissolved in excess 0.05N- 
NaHCO3, poured in shallow glass dishes, and placed in direct sunlight, or 
irradiated with a mercury vapor lamp. 6-hydroxypicolinic acid was detected 
only in the mercury lamp irradiated solutions (1198, 1204). 
4 Ow 
C1. c-Cl, a Sy (ton 
a N-Serve 
“A 
6 mee So 
cis y —OH ne 
6-Chloropicolinic 2-Chloropyridine 
acid 
a 
Cl NC AY Cl nN: “conjugates 
CH>-C-OH 
262 
