Ruelene [4-tert-butyl-2-chlorophenyl methyl methylphosphoroamidate ] 
Excretion and blood data, obtained after sheep were given oral doses 
of ruelene-P32, indicated that ruelene is rapidly absorbed and excreted. 
Eighty-five to ninety-five per cent of the dose was recovered in the 
excreta. Analyses showed that this compound was degraded to inorganic 
phosphate which was subsequently incorporated in bone. After incubation 
of ruelene with whole blood and plasma, chromatography showed the presence 
of dimethylamido phosphoric acid and phenylmethyl phosphoric acid. Several 
other metabolites were found but not completely identified (94). 
Sheep and poultry hydrolyzed both ester and amide groupings and formed 
a large number of innocuous hydrolytic compounds that were excreted via 
urine of sheep or urine and feces of poultry. One compound from sheep 
urine was believed to be 4-t-butyl-2-chlorophenyl dihydrogen phosphoric 
acid. A compound was isolated from insects that had a similar Re (66). 
Ruelene was fed to laying hens at a level of 100 p.p.m. for seven 
days. Six metabolites were isolated from excreta. Of these O-methyl- 
phosphoric acid and 4-tert-butyl-2-chlorophenyl methyl phosphoric acid 
were tentatively identified. Residues appeared in egg yolks 3 - 5 days 
after return to normal feed. Some tissues were residue-free after three 
days (213). 

o) 
a HH QO H 
"H 40-BN, o 
oH? WS cH Blood CH,0-B-N 
| OH “cu, 
lL -_ Plasma 
Hens Q 
oe ee a 
CH; 
O 
Ruelene + CH 0-8-oH 
er 
Sheep Poultry 
+ 4 Unidentified 
Compounds 

285 
