Trolene (Ronnel) [0,0-Dimethyl 0-(2,4,5-trichloropheny1) phosphorothioate ] 
After administration of radioactive trolene, the highest levels of 
trolene and its derivatives in all tissues were reached after 12 hours. 
Equilibration was reached after about 12 days. Residues persisting 7 days 
after oral administration were similar to trolene and its oxygen analog. 
The tissues in order of decreasing persistence of trolene and metabolites 
were those of: liver, kidney, spleen, subcutaneous and mesenteric fat, 
heart, brain. 
Trolene and its oxygen analog were hydrolyzed in rats and excreted 
in the urine initially as phenyl phosphoric and phosphorothioic acids. 
These decreased progressively while excretion of dimethyl phosphoric acid 
increased, 
When labeled trolene was fed to cows, the highest levels appeared 
in the fat, kidneys, and lungs. Fractionation of brain phospholipids 
showed some radioactivity in the lecithin-cephalin and sphingomyelin 
fractions. The peak level in the urine occurred from 18 to 32 hours 
after treatment. The predominant metabolite in the urine was identified 
as O-methyl-O-hydrogen 0-(2,4,5-trichlorophenyl) phosphorothioate. In 
rumen fluid, trolene was hydrolyzed to yield phenyl phosphoric acids. 
Some dimethyl phosphoric and phosphorothioic acids were also produced. 
Application of trolene to houseflies showed that the primary site of 
hydrolysis was at the phosphorus-oxygen-methyl bond as in rumen fluid 
(1il67):. 
Decomposition of trolene is normally base-catalyzed. When clays 
treated at elevated temperatures were used as carriers, a new type of 
decomposition occurred. After treatment at 950°C, all OH” is gone and 
the clay becomes a mixture of oxides. The decomposition product becomes 
one of molecular rearrangement (1238). 
329 
