TTD (Disulfiram) [Tetraethylthiuram disulfide] 
Plant tissues transformed N,N-dialkyldithiocarbamates into the 
corresponding glucosides (760) and ananine derivatives (761). By contrast, 
microorganisms produced aminobutyric acid derivatives (1304). 
In animals, enzymes converted TID to diethyldithiocarbamate, which 
in turn decomposed to CS5 (457, 741, 1179). In man CS5 was expired for 
4-5 hours after treatment and about 50% of the administered TTD was re- 
covered as CS» (987), Ninety percent of an S5°-labeled dose was absorbed 
from the intestinal tract and 10% was excreted in the feces, Analysis of 
urine indicated that about 65% of the sulfur was oxidized to sulfate and 
about 6% was recovered in the organic sulfur fraction (400). Excretion 
of unchanged TTD in urine is practically nil (373, 875); however, small 
amounts of both changed and reduced TTD were recovered from urine (400). 
The reduced form, diethyldithiocarbamic acid, has been detected in blood 
corpuscles and in alkaline urine but not in plasma (875). Recently, N,N- 
diethylthiocarbamoyl-1-thio-g-glucopyranosiduronic acid was isolated from 
the combined urine of four men given oral doses of TTD. Identification 
followed paper chromatography, isolation, infrared analysis and comparison 
of the melting points of the isolated and synthetic triacetyl methyl esters 
C759). 
Using $?°%-labeled material, a significant amount of the S35 was 
found attached to the serum proteins and to the soluble proteins of liver 
(1369). The greater part of this radioactivity was liberated by the 
addition of glutathione and the product was identified as diethyl dithio- 
carbamate (1368, 1370). In vitro studies also showed that TTD reacted with 
glutathione (740). 
Using rats, S?°-labeled TTD was administered intraperitoneally. The 
unchanged material was never detected in plasma, liver, or urine. Sulfate, 
CS,, the S-glucuronide of diethyldithiocarbamate, and traces of the free 
thiol were found in the urine, In addition to these metabolites, there 
were protein bound mixed disulfides in the plasma and liver (1369, 1370). 
Brea 
