

146 
It developes most rapidly on wort-gelatine, as described, but the 
growth is also good on sterilised blocks of pine, where it rapidly in- 
vades the cortex and cambium, and forms the typical wrinkled thallus, 
but with a pinkish-orange or flesh-coloured hue reminding one of a 
Tremella, and abounding in chlamydospores of the normal form and 
arrangement. As the growths aged they turned pink-brown. 
It also grows well on wort-agar, on agar with pine extract and Levu- 
lose, and on wort-gelatine with extract of cow-dung. ‘The development 
is distinctly inferior on agar with horse-dung, or with willow extract, 
and on pith saturated with pine-water. This is suggestive seeing that 
it grows very well on agar with pine-water and Levulose, and should be 
compared with the behaviour on agar with horse-dung and wort-gelatine 
with cow-dung. Clearly the presence of wort or a sugar such as 
levulose promotes the growth, whereas dung extracts obstruct it. This 
points to its being a fungus which developes on vegetable matter and 
not on dung or animal substances, and is quite in accordance with its 
origin from pine leaves. On the carbo-hydrates made up with yeast 
extract, mentioned above, the behaviour was as follows. 
The best growths were with dextrose, the next best with gum, and 
mere traces of development occurred in dextrine, lactose, xylose, 
mannite, maltose, or even saccharose. 
The effects of temperature came out very clearly in these cultures, 
and I verified them in cultures on agar media and in hanging drops. 
At 32°C no growth at all occurred ; at 22°C it was very slow and ap- 
peared not to take place above 24°C; at 16-18°C it was much better 
and apparently most rapid. 
No signs of fermentation were to be observed in any carbo-hydrate, 
and since levulose and dextrose appear to suit the fungus best we may 
conclude that the yeast cannot invert saccharose, maltose, lactose Of 
xylose. 
Although I have had the fungus in culture for a year, no further 
development has occurred on any of the media under any conditions 
tried, and we must conclude that it is able to go on repeating its de- 
velopment in the form described for indefinite periods. Whether it 
actually does this in the forest cannot be decided, but it is obvious 
that it may do so. 
Nevertheless I shall give reasons for believing that such would pro- 
bably not occur, but that in Nature some conditions are realised— 
which I have failed to imitate—which would impel the chlamydospores 
to grow out, or germinate and complete the development of what is 
here an arrested form. Moreover, I think there are very good reasons 
for expecting that the completed form would be a Tremella, and that 
in its present arrested condition it would go into Naematelia. 
Before doing this it is interesting to observe that the flat, yellow, 
wrinkled, and apparently full-grown discoid growths shown in Fig. 1 
were sent to several mycologists with the request that they would give 
me their opinion as to the identification of the fungus. This they 
were kind enough to do, and I take this opportunity of thanking them. 
All were very guarded in their replies, at which no one can wonder, 

