Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
Fig. 
149 
4, A single yeast-cell isolated in a hanging-drop of wort-gelatine 
was fixed (a) at 12 noon; at 9 p.m. it had budded off another 
cell (b); at 12 p.m. (¢) a second bud had formed at the 
opposite pole. Next morning at 9.30 a rapidly growing 
colony was formed (@), which at 2 p.m. (e) showed signs of 
outgrowing hyphe. At Io a.m. on the day following the 
much larger mass of budding yeast-cells was surrounded by 
a fringe of wavy hyphe, t. 18°C falling to 14-15° during the 
night. a-e x about 800; f x about 30. 
5. A similar series, @ at 12.20, b at 3 p.m., ¢ at 3.30, and @ at 
8.20 p.m. At ro a.m. next day the colony (¢) was putting 
out hyphz, clearly traceable to the yeast-cells. Culture in 
wort-gelatine at ordinary temperatures, as before. x about 
800. 
6. A similar series, showing still more clearly that the hyphee 
spring from the yeast-cells; @ at 12.30, Db at 3.10 p.m., ¢ at 
3.40, and d at 8.30 p.m.; ¢ at g a.m. next day, f at 4 p.m. 
Medium, temperatures and magnification as before. | 
», A similar culture, in wort-gelatine as before, but at a higher 
temperature—22-23°C. The yeast-cell was isolated at 12 
noon on Dec. 14, and at 11.15 a.m. on Dec. 15 had only de- 
veloped as in a; } shows its condition at 11.30 a.m. on Dec. 
16, and since it had only grown to the stage shown at ¢ by 
2.45, and remained stationary at 4.5 p.m., the temperature 
was reduced to 17.5-17°C. At 11.30 next morning (Dec. 
17) it was recovering its activity (d) and thence grew as 
rapidly as the specimens shown in Figs. 4-6. Magnification 
as before. 
8. A colony on wort-gelatine at ordinary temperatures on the 
fifth day. It is still actively growing, and has only just be- 
gun to form chlamydospores in the central older portions xX 
about 30. 
9. A colony the same age on wort-gelatine and extract of cow- 
dung. It has already finished its growth and has numerous 
ripe chlamydospores all over x about 30. 
10. A group of ripe chlamydospores from the last culture xX 
about 800. 
11. Irregular growth and imperfect chlamydospores forming at 
24°C x about 4oo. 
12. Extremely fatty contents of chlamydospores and yeast-cells 
from a three weeks’ culture on pith with pine extract and 
xylose. The growth was poor, and many irregular imperfect 
chlamydospores are forming. x about 800. 
13. A colony 3 days’ old in dilute wort-gelatine at 16°C from a 
yeast-cell which had been exposed outside and repeatedly 
frozen (on wort-agar from Dec. 29 to March 2). The growth 
was rapid and strong, and numerous chlamydospores were 
already formed on the older parts. about 50. 
14. Part of the margin of the same colony, showing the mode of 
