oo4 REPORT OF THE HARVARD AFRICAN EXPEDITION 
Nothing definite resulted in the monkey except a local suppurating lesion which 
healed rather quickly and in which bacteria were found. There were no other 
opportunities for the inoculation of animals at the time and no conclusions ob- 
viously can be drawn from this single experiment. A number of attempts to 
cultivate the organism were made. In the beginning the cultures were so over- 
grown with bacteria and molds that no opinion of value with reference to etiology 
could be obtained from them. Streaking a drop of necrotic material from a 
suppurating lesion on six successive slants of tubed media gave only a few colonies 
of cocci in the fifth and sixth tubes where the colonies were isolated. Finally, 
however, a piece of tissue was removed from a deeper portion of a lesion, and 
placed in a test tube containing ninety-five per cent alcohol for afew minutes; it 
was then washed in sterile saline solution and sectioned with a sterile knife. 
Cultures were made on Sabourraud’s medium from the knife and from the cut sec- 
tions of the tissue, as well as from crushed bits of the tissue. These cultures 
after thirty-six hours showed a few isolated colonies, but cultures made on potato 
dextrose gave no growth. The colonies which developed in the tubes on Sa- 
bourraud’s medium had at first a pinkish tinge, but they very soon became of a 
light brown color. 
Microscopical examination of the cultures showed both hyphae and spores. 
The hyphae were about 2 to 3u in width and were branching and septate. The 
spores were situated both terminally and along the sides of the individual hyphae, 
sometimes with a short sterigma visible. No chlamydospores were observed. 
The histological examination of many sections of the tissues shows that an 
extensive inflammatory process is present in the corium. There are many 
small abscesses scattered through it which are nodular in form (Illust. No. 
261). The center of these miliary areas consists of degenerated fibrous 
tissue which stains pink and uniform and contains epithelioid cells and is in- 
filtrated with many polymorphonuclear leucocytes. There are also, toward the 
periphery, many endothelial phagocytes which have engulfed the polymor- 
phonuclear leucocytes and round cells. In these necrotic areas spores of the 
fungi are found in good numbers. They are usually round, frequently single, 
and often dumb-bell shaped; more rarely in a clump of three or a chain of four 
elements. There is considerable variation in their size, the different forms 
measuring from 1 to 5u in diameter. Many are free in the tissue, but a few are 
seen within endothelial leucocytes. They are also observed in the lymph spaces 
and within the blood vessels or in an endothelial cell in the wall of the vessel 
(No. 259). 
These spores usually stain much deeper than the nuclei of the cells of the 
human tissue. 
Outside the zone of more acute suppuration is one densely infiltrated with 
endothelial leucocytes, small round cells and a few plasma cells, with fair num- 
bers of fibroblasts. Such zones are often surrounded in turn by areas of dense 
fibrous tissue. The endothelial cells of the vessels show proliferation which in 
places is quite extensive and some of them contain phagocytized leucocytes. 
In the epidermis, also, there are small abscesses containing numerous polymor- 
