SPOROTRICHOSIS 3a/ 
mycosis observed in Sao Paulo. In every case there were numerous parasites in 
the tissue which resembled tuberculous granulation tissue. The micro-organisms 
found were spherical and showed all sizes from exceedingly minute bodies, 
coccus-like, to 25 to 30u; these minute bodies surrounded the larger cells which 
were unstained; they were sometimes gram-positive or gram-negative. They 
regard the minute forms as probably spores by means of which these fungi 
multiply in the tissues. Drawings and photomicrographs show a central para- 
site surrounded by small spherical spores or granules. 
Regarding the differentiation of the different species of sporotricha isolated 
from human beings, Weidman! says that during the last eight years he has 
been able to collect seven strains of fungi causing blastomycosis. 
Filho * has recently isolated in Brazil, from a case with hard vegetating lesions 
covered with crusts, and associated with two small abscesses, a fungus which 
produced yellowish-white colonies on Sabourraud’s medium. He believes it is 
still another species, and has named it Sporotrichum fonsecat. 
In not all of the cases which resemble sporotrichosis clinically have fungi of 
the genus Sporotrichum (Rhinocladium) been isolated. 
Froilano de Mello and A. Rodrigues * have reported, with illustrations, a case 
of fungus infection in which the verrucose or pustulo-ulcerative lesions resembled 
those of our Liberian case. They, however, believe that the organism they found 
was a Blastomyces allied to the ‘‘type Gilchrist,’? but they remark that there 
was a total absence of giant cells and mononuclear infiltration in the tissue. 
DaFonseca, Area Leao, and Penido?* have isolated from cases with lesions re- 
sembling sporotrichosis a species of Hormodendrum in one instance, and in an- 
other a fungus identified as Hemispora stellata. Montpellier and Catanei°® have 
also isolated from cases with similar lesions in one instance a Blastomyces called 
Cryptococcus montpelliert, and in another a species of Hormodendrum, H. alger- 
iensis (see page 346). 
Finally, Da Matta ® has recently reported a case of ulceration with surround- 
ing nodules from which a fungus which he named Sterigmatocystis tropicalis was 
isolated. 
Many of the ulcerative lesions we observed in Africa contained spores of 
fungi. We not infrequently found such spores in the microscopical examinations 
of such lesions and also obtained them sometimes in cultures. In many instances 
it was impossible to say whether the fungi were present as saprophytic invaders 
or whether they constituted a secondary infection of the lesion which they ac- 
tively modified. 
Boucher ‘ in his extensive study of mycosis in the Ivory Coast points out the 
frequency of mycotic infection and found that in Bouake a third of his patients 
1 Weidman: Arch. Derm. and Syph. (1929), XIX, 875. 
2 Filho: Rev. Medico-Cirur. do Brasil (1929), XX XVII, 266. 
3 De Mello and Rodrigues: Bull. Soc. Path. Exot. (1929), XXII, 142. 
4 DaFonseca, Area Leao, and Penido: Comptes Rendus Soc. Biol. (1927), XCVII, 1772. 
5 Montpellier and Catanei: Bull. Soc. Path. Exot. (1926), XIX, 586; Ann. Derm. et Syph. (1927), 
VIII, 626. 
6 Da Matta: Bol. Inst. Brasileiro de Sci. (1928), III, 51. 
7 Boucher: Bull. Soc. Path. Exot. (1918), XI, 306. 
