436 REPORT OF THE HARVARD AFRICAN EXPEDITION 
Neveu-Lemaire ! notes that the wart hog (P. africanus and P. aethiopicus) 
can harbor the larva of Porocephalus annulatus, while Wenyon ? notes the infec- 
tion of P. aethiopicus with Trypanosoma brucei, T’. congolense and T’. simiae Bruce 
et al. 
Buffalo, Bubalus caffer; Bubalus nanus. In eleven buffalo of the larger Cape 
type, Bubalus caffer, shot upon the plains of Lake Edward and upon which 
necropsies were performed, in seven a species of amphistome was found in the 
stomach or small intestine; in three others no observations were recorded re- 
garding intestinal parasites, owing to unfortunate conditions such as darkness, 
distance from camp, ete., preventing a complete examination. In one animal 
it is stated that no intestinal parasites were found at the necropsy. However, 
it is realized that if very few parasites were present they might easily have es- 
caped observation in some portion of the stomach or intestine. It seems prob- 
able that in this region of the Congo, infection of this type of buffalo with this 
amphistome is not only very frequent but perhaps almost universal. 
The specimens of amphistomes were sent to Dr. Horace W. Stunkard, Direc- 
tor of the Biological Laboratory of New York University, who has kindly 
studied them and reports that the species is Cotylophoron cotylophorum (Fis- 
choeder, 1901), Stiles and Goldberger, 1910. Stiles and Goldberger * in 1910 gave 
a description of the genus Cotylophoron and of twenty species. Stunkard * has 
recently in connection with the examination of material from the Belgian Congo, 
carefully described the species Cotylophoron cotylophorum, in material from the 
domestic cow and from the stomach of the antelope Neotragus pygmaeus and 
from the stomach of another antelope, Adenota kob alurae. This trematode, 
though it was more frequently found by us not free but attached to the mucous 
membrane of the intestine, apparently produces no lesions. No evidence of its 
having bored into the intestinal wall was found and no cysts were seen in the 
alimentary tract. Also, histological examination of sections of the intestine of 
the buffalo which harbored this parasite, shows that the mucous membrane is 
normal. In one instance, however, there has been an extensive postmortem 
invasion of the intestine by a fungus which was evidently present in the intestine 
at the time the animal was killed. Very numerous spores of this fungus are 
found on the surface of the mucosa of the intestine and extending in smaller 
numbers through the mucosa and into the submucosa. There is no cellular reac- 
tion upon the part of the host about the cells of the fungus and it seems quite 
evident that the invasion of the tissues occurred after the animal was shot and 
during the few hours that elapsed before the necropsy could be performed and 
the tissues placed in the preserving fluid. These animals often were shot long 
distances from camp and hence considerable time sometimes elapsed before the 
bearer with microscope and laboratory equipment could reach the locality. 
A species of Setaria, subsequently identified as S. labiato-papillosa, was en- 
1 Neveu-Lemaire: Ann. Parasit. (1927), V, 368. 
2 Wenyon: Loc. cit., p. 511. 
* Stiles and Goldberger: Bull. Hyg. Lab. No. 60, U. S. Public Health and Marine Hosp. Service 
(1910). 
4 Stunkard: Bull. Amer. Museum of Nat. Hist. (1929), LVIII, 263. 
