438 REPORT OF THE HARVARD AFRICAN EXPEDITION 
There was also a leucocytosis of 18,000 and an eosinophilia of forty-two to sixty- 
two per cent. The patient was said to be cured by treatment with magnesium 
sulphate, increasing doses of hexamine with bicarbonate of soda, and intra- 
venous injections of stibosan. 
A herd of Ituri Forest buffalo suddenly charged toward us in high grass, and 
unfortunately in the rapid firing necessary to stop the charge two cows were 
killed, one a pigmy black and one a marginal black. These two cows were found 
to be pregnant and in the uterus of each was an apparently almost full-term calf. 
In each instance the hair of the foetus was jet black, like that of the mother. 
Mention is made of this fact because it has been stated by several zoologists and 
hunters that the newly-born calf of the marginal black type is red and that the 
hair turns black only in the older animal. 
In one buffalo, an old animal, there were found at autopsy in the liver small 
grayish-white areas from pinhead to about 5 mm. in diameter. Upon aspiration 
with a small syringe they were apparently small cysts containing clear, very 
albuminous fluid which coagulated quickly in aleohol. They seemed to possess 
a distinct cyst wall. Microscopical examination of the contents did not reveal 
any parasites or ova. The portions of the liver containing the cysts (?) were 
preserved in Zenker’s solution. Histological examination of these tissues shows 
that these circular spaces in the liver or cysts are lined with a thin membrane 
and that there is no cellular reaction whatever, inflammatory or otherwise, in 
the surrounding tissue. The surrounding liver cells appear perfectly normal. The 
cysts or sacs are empty. There is no evidence whatever that they are of para- 
sitie or infectious origin. 
In one of the buffalo two small fibroid tumors were found in the skin and in 
another a small abscess about 5 cm. in diameter on the back. The microscopical 
examination of the abscess showed both bacteria and fly larvae present in the 
abscess. The sections, however, show no fly larva, though both cocci and bacilli 
are present. 
A large number of ticks, of tsetse, and other flies were also collected from 
a number of these animals. They are reported upon by Bequaert in the entomo- 
logical section of this Report (Chapter XXXVI). 
Of eight buffalo examined by Bruce, Bubalus caffer, the blood of one when 
injected into a dog was said to have caused infection with Trypanosoma brucei. 
Neveu-Lemaire ! therefore, lists these animals as a reservoir of the virus of 
nagana in Zululand. We were unable to find trypanosomes or other parasites 
in the blood of the animals we shot. 
In addition to the parasites of man already mentioned, that have been re- 
ported in Bubalus, the cysticercus of Taenia saginata? and T. solium and the 
“hydatid” of Echinococcus granulosus are to be included. Possibly the buffalo 
may also be a host of Fasciola gigantica. 
Hippopotamus amphibius amphibius. In the examination of eight hippo- 
potamuses which were shot in the Ruchuru Plains near the Ruchuru River and 
1 Neveu-Lemaire: Ann. Parasit. Humaine et Comparée (1927), V, 150. 
2 Cameron: Proc. Royal Soc. Med. (1927), XX, 547. 
