a 
Introduced Sapeet ease Tinla popilliavori.--M. H. Bruneeey ‘Nocteae 
town, reports that in an effort to ascertain reasons for the failure of 
colonies to become established in the field, laboratory observations this 
year have been devoted primarily to the interrelation of the parasite and 
host and to the mating of the parasite. Evidence obtained in 1932 indi- 
cated that females collected early in August, the period in which adults 
appear in the field, were not fertilized; as the progeny of these females - “4 
in the laboratory were largely males. In 1932 cocoons were obtained from 
females seen mating and females placed with males for 1 and 2 days, for — 
comparison of their progeny in 1933. The grubs used in this work were, 
as much as possible, used no more than 1 day after’ being dug in the ‘field. 
The results are as follows: 
| Cocoons yield~ 
Source of cocoons in 1932 Cocoons Adult emergence 1933 ing adults 
Number Males Females Percent 
Fenales séen-mating. Host 
second: and third instar a, . 
grubs e e ° * e 2 . - s e é 678 241 ; 194 ; : 64. rH 
Females with males for 
one day for ‘nia tirig. Host ee 
second instar Brus. eer 152 114 a 76.9 
Fenales with males for 
one day for mating. Host 
third instar grubs ... SYA PORE SS ASE 269 69.2 
Females with males for 
two days for nating. Host 
second and. age instar : Bice The Te ee ee 
ETUDS ot a ee ene 9 454 160 7 BS Ba 
The results obtained: this year indicate not only that mating of fe- 
males is necessary for the progeny to be part females, but that the nun- 
ber of females appearing in the progeny of mated females may be greatly 
influenced by the host. When second-instar grubs are parasitized, the 
progeny may be largely males, but the progeny from third~instar grubs 
will be about even males and females, or largely females. Observations 
this year revealed that unmated females mated, even after ovipositing for 
9 deys: that practically all, if not all, of the females collected in the 
‘ied mated; and that females mate but once. 
Emergency relief labor.--Labor has been furnished by the local relief 
administrator in connection with the Emerz gency Relief Administration for 
Now Jersey to the extent of 238 man days during the month of October for 
sda work at the Moorestown laboratory. During October at the 
vbury, long Island, laboratory 520 man hours of Jaber have been fur- 
nisacd es the Emergency Relief Administration for Asiatic garden beetle 
zruo surveys in New York. 
