
a ee 
that the total loss in weight of €C. pomonella was 3 per cent: of their 
water content they lost 1 per cent; glucose, 33 per cent; glycogen, 10 
per cent; fat, 15 per cent; insoluble nitrogen, 0.3 per cent; insoluble 
protein, 0.65 per cent; soluble nitrogen, 0.05 per cent; and soluble 
protein, 0.14 per cent. For A. comptana, on the other hand, the total 
loss of weight was 15 per cent; the loss of water, 2 per cent; glucose, 
8 per cent; glycogen, 26 per cent; insoluble nitrogen, 0.05 per cent; 
insoluble protein, 0.25 per cent; and soluble protein, 0.01 per cent. 
They lost no soluble nitrogen and they gained 0.5 per cent fat. 
J. W. Bulger, Takoma Park, reports that with the assistnce of 
Abby Holdridge, he has "calculated a table which shows that 15 tubular 
cutters ranging in size from .14 to .28 inch in diemeter, inclusive, 
will vary the size of sandwich sufficiently to enable us to feed silk+ 
worms ranging in weight from .30 to .39 gram, respectively, doses of 
poison which will, according to calculations, approximate as closely 
as possible any given median lethal dose. This will enable us much 
more rapidly and accurately to establish any given point on the tox- 
icity curve for a given poison or substance. This method should prove 
to be a substantial improvement over our present sandwich method." 
Reporting further on the studies by himself and W. N. Sullivan on 
the toxicity of rotenone to house flies, F. L. Campbell, Takoma Park, 
states that there is no appreciable difference in the susceptibility 
of the sexes to rotenone in acetone at l1+to-5,000. Tests were made 
also to compare the results of treatments in the cold room with those 
at room temperature. It was found that "an average of 80 per cent of 
the flies treated with rotenone 1—to-5,000 in acetone died in 48 hours. 
Since the same solution kills only about 40 per cent of the flies that 
are treated in the cold room, it must-be concluded that the treatment 
is more effective at room temperature." Dr. Campbell also states that 
in his rearing work, "The dying out of the maggots * * * has been pre- 
vented by reducing by one-third the quantity of water in the medium. 
Puparia are now formed below the surface of the medium, and the mature 
maggots no longer crawl to the top of the jars. It is worth noting 
that the temperature of the medium at the bottom may rise to 117° F. 
during the initial fermentation without injury to the maggots. Egg — 
production continues to be satisfactory, although the flies have not 
been exposed to sunlight for three months." 
Since the controversy about the location of the olfactory organs 
in insects still remains unsettled, N. E. McIndoo, Takoma Park, tested 
blowflies, Calliphora erythrocephala Meig. and Phormia regina Meig., in 
his wooden olfactometer and found no difference between the olfactory 
responses of unmutilated flies and flies with their antennae pulled off. 
Each set of flies consisted of about 200 insects and in regard to their 
behavior and mortality there was not a great difference between the un= 
mutilated and the mutilated flies. 
