74 STORRS AGRICULTURAL EXPERIMENT STATION. 
This is probably explained by the fact of the great development 
of Group IV. instead of the acid forming Group I., since the 
development of acid commonly checks the growth of bacteria. 
The miscellaneous species developed in this case, unhindered 
by such acidity. It will be seen, however, that the liquefying 
bacteria, Groups VI. and VII., were reduced very greatly in 
percentage, although they actually increased in numbers for 36 
hours. 
The sample of Milk kept at 1°, as already stated, was unfor- 
tunately removed from the cold storage after three tests were 
made. ‘These three tests, as given in the tables, only show that 
during 148 hours, at 1°, there was practically no change in the 
bacteria in the milk, the numbers remaining nearly constant, 
and the percentage of each test remaining about the same. One 
or two of the species originally present in small numbers were 
not found in the third test, and had very likely disappeared. 
EXPERIMENT NO. 8. DECEMBER 4. 
In this experiment the conditions were an exact repetition of 
those of the last experiment. The milk kept at 20° curdled in 
48 hours, just at the time of making the last test. Milk kept | 
at 10° did not curdle, but became somewhat thick after 240 
heurs. ‘The tests were carried on for some time after this, and 
the last test was made at 408 hours, when the milk was thick 
but not curdled. The milk kept at 1° was tested at varying 
intervals, as shown by the table, for 42 days. Atthe end of 42 
days it was not curdled, although it was somewhat thick. ‘The 
experiment showed a number of peculiarities which may best 
be explained in connection with the tables which contain the 
data. 
The most peculiar and interesting feature of this experiment 
was the appearance in all samples of a species of bacterium which 
had not been found before, and which apparently modified the 
results in a very extraordinary way. This species belonged 
to Group IV., but when the detailed characteristics were studied 
it was found to be a species not hitherto described, and it was 
tabulated in our listas No. 249. It was an organism that grows 
well at low temperatures, but does not grow at high tempera- 
tures. It produced no acid reaction, but, on the other hand, 
