




CLASSIFICATION OF DAIRY BACTERIA. A7 
Gelatin stab; needle growth abundant, with not much surface at first. Later 
a deep, dry, narrow pit is formed, with a dense, white growth covering its 
walls. Still later the gelatin liquefies, and a yellowish sediment is produced. 
Agar; opaque, abundant growth, with a tinge of yellow. 
Potato; rather dry, whitish or with a yellowish tinge. 
Bouillon; becomes uniformly cloudy, with no scum and with a s/zmy sedi- 
ment. Another culture of what I regard as the same species produces a liquid 
pit instead of a dry pit, as here described. 
Milk; culture does not curdle milk. This is the only one of the liquefying 
cocci found that fails to curdle milk. 
I regard this as identical with No. 117 of Group IV. 
Nona? ’(Rate. ) 
Morphology; a minute cocci, .3¢ to .4m@ in diameter. 
Gelatin plate; a small, granular colony, surrounded by a clear, liquefying 
ring. Later the nucleus breaks up, diffusing through the liquid, sometimes 
regular, sometimes irregular. 
Gelatin stab; growth is slow. A narrow pit is formed, with an air bubble at 
the surface. Liquefaction complete in six weeks. Liquid is cloudy, with a 
heavy sediment. 
Agar and potato; dirty white growth. Not characteristic. 
Milk; is curdled, with weak alkaline reaction, in two days, at room tempera- 
ture. At 36° in three days. A digestion follows, which is never complete. 
Chiefly characterized by its mznufe size. Found only once. 
No. 109. — (Rare.) 
Morphology; a coccus, Tm in diameter. 
Gelatin plate; a round, smooth surface colony, moderately transparent. 
Grows to the size of 1.5 mm., usually very flat, with a central mound, and 
‘then sinks into a pit, upon which it forms at first a very dense surface scum. 
Gelatin stab; slight needle growth; a very shallow, dry pit is produced at 
first, in which liquid begins to collect after four or five days. Liquefaction 
produced very slowly in a horizontal layer. 
Agar and potato; not characteristic. 
Milk; is curdled at body heat in seven days, quite solid, and strongly 
alkaline. At room temperature it digests without curdling. The liquid is at 
first watery, soon becoming amber color, and later the color deepens, some- 
times even to a mahogany color. When used for cream ripening no effect is 
produced on the butter. 
No. 119. (Rare.) Sarcina alba (?) 
Morphology; a sarcina form, .64 to .8u in diameter. 
Gelatin plate; a slightly yellow, round, raised, opaque colony, which soon 
sinks into a pit. The pit remains clear, with a rough, granular nucleus. 
Gelatin stab; a very narrow liquefying pit is produced, with a cloudy liquid. 
This broadens below the surface. Sometimes, however, a shallow, deep, dry 
pit is formed. 
