58 STORRS AGRICULTURAL EXPERIMENT STATION. 
a few lobe-like shoots extend from the colony into the gelatin, exding in 
prominent knobs, quite characteristic. 
Gelatin stab; a shallow cone produced. The gelatin then liquefying regularly 
into a dense, cloudy liquid. 
Agar and potato; scanty growth, but not characteristic. 
Milk, is curdled hard and rendered amphoteric, or sometimes acid. No di- 
gestion can be seen, but a watery whey subsequently deposits from a solid curd. 
Nos. 176 and 189. &B. varians lactis II. and I/1. 
These two cultures I place with No 196. They agree in all points except 
the power of liquefying gelatin. No. 139 in gelatin stab forms a deep, dry 
pit, with no sign of liquid. No. 176 produces a dry pit, but later begins to 
liquefy at the bottom, and the liquefaction slowly deepens, while No 196, as 
shown, liquefies rapidly. The three were obtained from different localities and 
at different times. 
They may be identical with 2. cloacae (Jordan). 
No. 64. (Rather common.) 2B, circulans II. (n. sp.) 
Morphology, size, .64 by 1.54. Long chains are produced in bouillon, but 
no spores found. 
Gelatin plate; a granular bead is produced, which sinks into a dry pit. The 
pit liquefies, and the bacilli can be seen actively circulating in the liquid. 
Uniformly granular. 
Gelatin stab; there isa growth along the needle track, producing a deep, 
narrow funnel, from which the liquid evaporates, so that there is a consider- 
able portion of the funnel without liquid. A white sediment forms in the axis 
of the liquefying pit. The whole is peculiar and characteristic. 
Agar, an abundant, yellowish growth. Not characteristic. 
Potato; a somewhat thin, watery, transparent growth, 
Milk, there is no curdling, but the milk digests into a weak alkaline liquid, 
which is cloudy and gives off unpleasant odors. Butter made from cream 
ripened with this organism develops a moderately good flavor and aroma. The 
putrefactive odor in the milk and cream is ordinarily not noticeable in the 
butter. 
This is very similar to B. cérculans of Jordan. I have, however, found no 
spores, while Jordan found them in most media.. The growth on agar is also 
different. The peculiarities shown in the colony and the gelatin stab lead me 
to put it with the species described by Jordan as a Variety LT, 
No. 164.: (Rare.) 
Morphology, size, .54 by .8u to Im, Tapering ends and showing irregular 
stain. 
Gelatin plate, a yellow colony in a deep pit. As liquefaction begins there is 
a curiously figured central lobate mass, with an outer clear zone. 
Gelatin stab, a shallow, dry pit appears, which soon shows liquefaction at 
the bottom, an air bubble remaining for some time. Later liquefying over the 
whole surface into a cloudy liquid, with a dense sediment. 

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