

CLASSIFICATION OF DAIRY BACTERIA. 65 
Potato; a ground glass, much folded layer is produced with liquid under the 
folds. Later the folds increase and the whole becomes white. 
Milk; curdles rapidly at 20° and 36° into a hard curd and no whey. Alka- 
line. Digests into a clear, yellowish liquid. 
Division B. 
Spores at one end of the rod. 
No. 189. (Rare.) 2. arborescens lactis IJ, (n. sp.) 
Morphology, size, .8u by 4u. The spore is Im by I.2u. Occasionally two 
or three rods together, but no chains. 
Gelatin plate; colonies become I cm. in size or larger, showing radiating 
fibers strewn with knots. ‘Vhe fibers themselves are fine and branching. The 
knots look like isolated colonies and each frequently shows secondary radiation. 
Fibers grow mostly under the surface. Very characteristic. 
Gelatin stab; an arborescent growth underneath the surface of the gelatin. 
Branches extending horizontally from the needle track and ending in knobs. 
In two days a dry pit is formed on the surface with numerous disjointed colo- 
nies extending from this pit to the edge of the tube. Later liquefies at the 
surface with a dense white cloudy liquid. 
Agar, surface curdled completely with a thin, hardly visible growth. 
Potato; thin and scanty growth. 
Bouillon; a tough scum is formed which sinks while disturbed and forms a 
flocculent sediment. Later the scum sinks and the liquid is slightly cloudy. 
Milk, no effect produced upon milk. 
Division C. 
Bacilli with two spores, one in either end, 
The following species is certainly peculiar. The rods are very long, from 2u 
to 6u, and in each end of the rods may frequently be seen a clear unstained 
body. I have regarded them as spores, even though it has generally been 
thought that a bacillus with two spores has not been found. At all events this 
species is very unlike any other found and may be most easily recognized from 
this apparent double spore formation. I have therefore placed it in a division 
by itself with a distinct specific name. 
No. 190. (Rare.) &. dtspora lactis. (n.-sp.) 
Morphology, size, 1.84 by 24 to 64. Spores are about tu to 1.5m, variable 
in size. Long chains of threads are formed with rounded or tapering ends, like 
a string of sausages. 
Gelatin plate; a round, tough colony is produced, yellowish in color, easzly 
removed intact by a platinum loop. When reaching the size of I mm. it sinks 
into a slowly liquefying pit, but the mass of bacilli remain as a distinct nucleus 
for a long time, the liquid being clear. 
Gelatin stab, ashallow funnel is formed and the liquefaction becomes complete. 
Agar and potato; not characteristic except that both show a tendency to have 
isolated clumps rather than a uniform layer. 
Milk; at 20° is curdled and rendered amphoteric or alkaline. A slight 
digestion is apparent. 
