118 STORRS AGRICULTURAL EXPERIMENT STATION 
FIFTH TREATMENT OF THE MACHINES. 
The manufacturers of these machines have recommended 
the use of strong brine as a means of keeping the rubber tubes 
sweet and free from bacteria between milking times. During 
the experiments given in Table 30, the machines were washed 
and the pails steam sterilized as before. But all the rubber 
tubes and the teat cups were allowed to lie in a 10 per cent 
salt solution between milkings both day and night. | 
By testing the rubber tubes thus treated, it was found that’ 
the salt solution was not keeping them free from bacteria. 

Fic. 42a—Plates showing numbers of bacteria in water used for rinsing 
inside of rubber tubes which were treated with brine solution. .1 cc. 
and .5 cc. of rinse water used in plates. Large white spots are 
liquefied areas. Each black spot is a colony of bacteria. Large 
black spots are molds. 
