I20 STORRS AGRICULTURAL EXPERIMENT STATION. 
followed at the opening of each new can, and the composite 
sample thus obtained, after thorough mixing, was analyzed as 
representing the composition of the material. 
Samples of the cod were taken from each portion fried, care 
being taken to secure a representative sample in each case. 
The mixture of the samples thus obtained was then analyzed. 
The sample of duck was obtained by removing the meat 
from the cooked birds and-carefully shredding it into as small 
pieces as possible. After thorough mixing, a portion was then 
removed for analysis. 
The milk and bread were obtained fresh each morning, and 
aliquot portions of each were taken. ‘The samples for all the 
days of an experiment were then mixed together and formed a 
composite sample for analysis. In the case of the milk, a few 
drops of formalin were added to the composite sample as a 
preservative. 
Since the butter was purchased in amounts sufficient for an 
entire series of experiments, a sample was taken at only the 
beginning of each series. A single sample of the duck-fat was 
likewise sufficient. 
No analyses were made of the sugar or rock-candy, as these 
materials are known to be of uniform composition. 
Separation of feces.—In these experiments, separation of the 
feces pertaining to the experimental period was effected by 
means of lamp-black in gelatin capsules, taken with the first 
meal of each experiment and the first meal following the com- 
pletion of the series, according to the method explained in 
detail in a former report.* In most cases the separations were 
quite satisfactory, though in some cases the line of demarcation 
in the separation at the end of an experiment was not so 
sharply defined as is desirable. 
The feces pertaining to each experiment were collected, dried, 
weighed and ground for analysis. 
Collection of urine.—The urine was collected for twenty-four 
hours of each experiment, beginning at 7 A. M. of the third day 
and ending at 7 A. M. of the day following. In the absence of 
any means of separating the urine pertaining to the experi- 
mental period and the lack of definite knowledge concerning 

* Storrs Expt. Sta. Rpt. 1904. 
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