CLASSIFICATION OF DAIRY BACTERIA. I5I 
Bouillon.—A red sediment, a ring-like pellicle, and slight turbidity are 
formed. [Greenish. | 
Milk.—Rendered slightly alkaline, or there is no change in the reaction. 
No curdling nor digestion occurs, and there is no change in color. 
Potato.—A filiform, raised, contoured growth, of a brownish-yellow to an 
orange color; luxuriant, and with the potato discolored. [Yellow to brown, 
and sometimes folded. | 
Grows at both 20° and 37°. Aerobic. 
Variety B.—Isolated from milk here, is a similar, non-liquefying fluorescent 
monotrich, which we regard as the same. It is quitecommon and agrees closely 
enough with the above to be called by the same name. It commonly makes 
the gelatine green, and has a strong fluorescent action on agar. Milk is also 
turned green, but is otherwise unchanged. On agar the growth is rather 
scanty, but is luxuriant on potatoes, of a brown color to white. On potato it 
may be folded. 
Ps, lactis Estenit n.s. A Pseudomonas with a smoky fluorescence. This 
organism is quite common in milk. Another one practically identical agrees in 
all points except that it does not produce the smoky fluorescence. 
Morphology.—Size, .8u4-1.24 x .4u@. No chains are formed, no spores nor 
capsules, and the organism does not accept the Gram stain. 
Gelatine-colony.—A round, smooth, capitate, contoured, homogeneous colony, 
of a cream-white to a gray color. On /tmus gelatine it forms a pale; thin 
surface colony. 
Gelatine stab.—A filiform, needle growth, and a raised, dry, not spreading, 
surface growth. 
. Agar streak.—Filiform, raised, smooth growth, translucent; gray in color; 
luxuriant and slightly viscous. The agar becomes smoky. 
Fermentation tubes.—No acidity, gas, nor closed arm growth in any sugar 
bouillon. 
Bouillon.—A sediment and flocculent turbidity are formed, but no pellicle. 
Milk.—No action on milk. 
Potato.—A filiform, thin, smooth growth; gray in color; moderately luxuriant. 
Grows both at 20° and 37°. Aerobic. 
Variety B differs from A.in being slightly larger, 1.8u-3u x .7u, and in pro- 
ducing no fluorescence. 
Ps. lactis filiformis n.s. A yellow, non-liquefying Pseudomonas, Found 
once in New York milk. 
Morphology.—Size, 2.5¥-3.54 x .84-.9H. No capsule, and no Gram stain. 
Spores are produced which are frequently seen as long chains. One long 
flagellum. 
Gelatine colony. —A round, convex, smooth, entire colony of a creamish or 
yellow color. On “tmus gelatine the colony is red-brown, non-acid. 
