

E72 STORRS AGRICULTURAL EXPERIMENT STATION. 
Boutllon,.—A slight sediment and turbidity, but no pellicle. 
Milk.—No action. 
Potato.—A very scanty white growth, with discolored potato. 
Grows at 20° and very slightly at 37°. Facultative anaerobic. 
B. lactis mycoides n. 8. Rhizoid, spore-bearing bacilli. This type of bacillus 
we have found very frequently. There are some variations in the characters of 
the various cultures. The one described below, which we take as a type, was 
from Dr. Maher, and has been used by him in numerous inoculation experi- 
ments against certain diseases. The variations which we have found in other 
cultures are indicated in brackets. . 
Morphology.—Size, u-4u x .6u-1.2u. Long chains produced, spores present, 
no capsule, and Gram stain positive. 
Gelatine colony.—A small burr like, rhizoid colony, soon liquefying and form- 
ing a pit with anucleus. [The rhizoid character is not always found, and the 
liquefaction may be slow. The color is sometimes yellowish; it sometimes 
shows tangled threads like anthrax. ] 
Gelatine stab, An arborescent needle and a cratiform liquefaction. [Infun- 
dibuliform without arborescence. | 
Agar streak,—Luxuriant, dull, wrinkled, white, tough. [ Yellowish. ] 
fermentation tubes.—No acidity, gas, nor closed arm growth in any sugar . 
bouillon. 
Bourllon,—A sediment, turbidity, and a pellicle. 
Milk.—Rendered alkaline and curdled after a few days. Digested into an 
amber colored or yellowish liquid. ; 
Potato.—Luxuriant, velvety, dry, wrinkled, white. 
Grows at 20° and 37°. Aerobic or facultative anaerobic. [A ground-glass 
like appearance, with liquid under the folds. Sometimes of a pasty consistency. | 
LB. subtilis. Is extremely common in milk, though néver in great numbers. 
While it will grow in sterilized milk, it does not usually thrive in milk contain- 
ing lactic bacteria. In old milk it is, therefore, usually overgrown by the lactic 
organisms. We describe a typical culture and two varieties below. 
B. subtilis (Ehrb.). 
Morphology.—Size, 1.5u-A4m x .6u-1.5 1, commonly forming chains. Spores 
are produced in abundance. The Gram stain is positive, and there is no Cap- 
sule. The size is somewhat variable in different cultures. 
Gelatine colony.—A rapidly liquefying colony, with irregularly distributed 
granular masses. The appearance of these masses is striking but not uniform, 
and hence not characteristic. 
Gelatine stab.—Begins to liquefy in one day, crateriform and later stratiform. 
Argar streak.—A filiform or spreading, raised, contoured, cretacious, white, 
growth, frequently wrinkled and dull. In some cultures it is quite dry. 
fermentation tubes.—No acidity, gas, nor closed arm growth, in our cultures, 
although Chester says dextrose is made acid. 
