180 STORRS AGRICULTURAL EXPERIMENT STATION. 
Fermentation tubes.—Dextrose is acid, the other two sugar bouillons alkaline. 
Growth in closed arm in all cases, and no gas. [Dextrose and saccharose 
alkaline; lactose acid. ] 
Bouillon.—A sediment, a granular pellicle, and no turbidity. [Turbidity.] 
Milk.—Becomes alkaline, curdles and digests with a strong odor. [Acid, 
otherwise as above, except that the milk is brownish. | 
Potato.—Luxuriant, rather thick, rough, white. 
Grows at 20° and 37°. Facultative anaerobic. 
Variety A.—Agrees in all points except that the mold-like colony is not evi- 
dent, the colony liquefying very rapidly. 
II. No Pigment, but Acid in Dextrose and other Sugars. 
We have found at least three Aguefying gas producers.. ‘Two of them are 
quite similar to B. cloacae, while the other is closely related to it. The char- 
acters as made out by us are as follows: 
B. lactis cloacaen.s. A gas-producing, liquefying bacillus. 
Morphology.—Size, Iu-1.3u x .7@. No chains are formed. The organism 
accepts the Gram stain, and has a capsule, but produces no spores. 
Gelatine colony.—A slow liquefier, forming a dense granular pit. In /tmus 
gelatine. there is no acid. 
Gelatine stab.—Begins to liquefy in one day and is completely liquefied in 
six days. Liquefaction deep infundibuliform. 
Agar streak,—Filiform, raised, smooth, cream-white or yellowish, iridescent, 
luxuriant. 
Fermentation tubes.—All three sugars rendered acid and show growth in the 
closed arm. Gas produced in dextrose and saccharose only. 
Bouillon.—A sediment, turbidity, and a pellicle. 
Milk.—Rendered acid, curdled, and subsequently digested at 20° but not at 
37°, showing a yellow color. 
Potato.—A scanty, thin, irregular, white growth. 
Grows better at 20° than at 37°. Facultative anaerobic. 
B. lactis cloacae A.n.s. This was found in milk in this vicinity, but an 
almost identical organism was sent us by Weigmann from Kiel, labeled aerog- 
enes. It is clearly not that specie, since it liquefies gelatine. Its characters 
are as follows: 
Morphology.—Size, 1.5@ x .5u¢-.64. Chains are formed in bouillon. It pro- 
duces no spores nor capsules, and does not accept the Gram stain. 
Gelatine colony.—Round, raised, smooth, grumose, with wavy edge, gray- 
white. On “tmus gelatine a good sized, white, acid colony, 
Gelatine stab.—A rapid liquefier, infundibuliform, and showing an abundance 
of gas. 
Agar streak,—Filiform, flat, smooth, moist, not abundant. 
