50° STORRS AGRICULTURAL EXPERIMENT STATION. 
NO. '2. 
Locality.—Found in milk in Middletown, October, 1892, and also in ripened 
cream in Ellington Creamery, November, 1892. 
Morphology.—A micrococcus, size I . to 1.2 #. It never forms chains, 
Grows the same on potato and agar. 
Motility.—No motion. 
Relation to Air.—Growth under mica, none, or very slight. 
Colony on Gelatine.—A slight pit appears, which begins to liquefy. The 
colony at this time is smooth and uniform. As it grows it breaks up, resulting in 
a liquid colony with a somewhat clear center, which may contain few granules, 
and a periferal ring with dense granules. Sometimes the ring is not at the edge 
and is very dense and distinct. 
Gelatine Stab Culture.—Liquefies into a shallow pit with a thick granular 
yellowish scum. Liquefies slowly, only a quarter of an inch of the gelatine 
being liquefied after several weeks. 
A gar-Avgar.—A dry, raised, discreet streak of a salmon or Naples yellow color. . 
Surface is wrinkled, rather tenacious and sticky. 
Potato.—A thick, dry mass, which occasionally spreads slightly or sometimes 
forms a series of distinct beads. Rough and wrinkled. Color varies from white 
flesh color to a decided brown. Frequently salmon color. 
7 emperature.— Grows equally well at 20° and 35°. Color not so well devel- 
oped at 35°. ; 
Milk.—At 20° or at 35° milk is coagulated. Coagulum rather hard with little 
whey. Orange masses on top. Odor, sour. Alkaline reaction, at least at first. 
No digestion of the casein occurs. Chemical analysis shows the presence of 
butyric acid and alcohol. 
Bouillon.—Liquid clear, with a slight precipitation and no scum, Later, 
liquid becomes cloudy and a slight scum forms. 
Two varieties of this organism have been found, differing in the brilliancy of 
the pigment only. 
No. 5. 
Locality.—Ripened cream from Cromwell creamery, October, 1892. 
Morphology.—A large rod 2 py. by I y., never forming chains. On agar it 
is somewhat smaller than on gelatine. No spores observed, occasionally a ten- 
dency toward a bi-polar stain noted. 
Motilty.— 
Temperature.—Growth slight at 35°, and green color not produced. 
Relation to Aivy.—Will not grow under mica. 
Colony on Gelatine.—A white colony which sinks into a. pit with a granular 
center and a clear rim, spreading into a round, uniformly granular circle with a 
radiating rim and a greenish tinge. 
Gelatine Stab.—Liquefies slowly into a broad funnel, with a thick, tenacious 
scum. Liquid green and later yellow. 
Agar-Agar.—Smooth, moist, rough growth which becomes abundant after a 
few days and is very sticky. Agar turned green. 
Potato.—An abundant white growth with yellowish tinge spreading over the 
surface of the potato. Smooth and glistening. Sometimes it forms mounds, 
sometimes a rough, folded mass with pits. 

Ale a 491 I") 

