

BACTERIA IN THE DAIRY. 51 
Milk.—Does not curdle at 20°, but turns slowly brownish. At a higher tem- 
_ perature it may form a soft curd with a cloudy whey. Reaction amphoteric or 
alkaline. Is slowly digested into a lemon yellow, cloudy liquid. 
Boutllon.— Uniformly cloudy with abundant sediment. Liquid green. 
Nos. 16 AND 162. 
The two varieties named, 16 and 162, resemble each other very closely in their 
specific characteristics. It was found impossible to distinguish between them, 
except by their effect upon butter. In all the experiments 1672 produced butter 
which was decidedly sour, while the butter produced by 16 was less sour and 
was usually regarded as excellent. The characteristics are as follows: 
Locality.—Ripened cream from Mansfield, October, 1892. 
Morphology.—An oval rod or micrococcus about I y. in length. 
Motilty.—Stationary. 
Relation to Atr.—Grows well under mica. 
Temperature.—Grows profusely at 35° as well as at 20°. 
Colony on Gelatine.—F¥orms first a white bead, which spreads rapidly over the 
surface and is slightly iridescent. Sometimes forms a lobular colony with a 
raised central nucleus; sometimes there is an outer clear, transparent rim with a 
raised center. In six days colony reaches dimensions of 2 mm. 
Gelatine Stab.—An abundant needle growth with a raised, round, smooth 
surface growth. Gas frequently produced in gelatine. 
Agar-Agar.—A diffused, white, shining growth. Sometimes quite thin, at 
_ other times thick with a sharp edge. 
Potato.—Very abundant, thick and spreading, white with an irregular surface. 
Milk.—Curdles at 35°. Surface with a clear whey, which becomes frag- 
mented and full of bubbles. Does not curdle at 20°. Is strongly acid and 
shows no digestion. 
Bouillon.—Produces a uniformly cloudy liquid with a sediment and ‘a slight 
scum round the edges of the tube. 
No. 18. 
Locality.—Mansfield, October, 1892. 
Morphology.—A bacillus 2 y. by 1.4». Occasionally two or three may be 
seen attached together. Produces spores in its potato growth. Growth on agar 
the same, though slightly larger. 
Motility.—No motion. 
Relation to Air.—Grows under mica. 
Temperature.—Grows profusely at 35° and at 20°. 
Colony on Gelatine.—A white bead which spreads slightly and forms a round, 
smooth, glistening colony, about I mm. in diameter. 
Gelatine Stab.—Abundant growth along the needle track. A white nail 
growth on the surface which spreads in a rather dry layer. 
Agar-Agar.—A white, scanty, narrow streak along inoculation line. Some- 
times spreads. Not very characteristic. 
Potato.—Spreads abundantly into a thick, white, diffused, mottled layer, some- 
times raised into mounds. Later it turns decidedly brown and more uniform. 
Bouillon.—Produces a uniformly cloudy liquid with a sediment. A slight 
scum appears after several days. 

