a 
; gs: 
, aye 
BACTERIA IN THE DAIRY. 53 
Temperature.—Grows well at 35°, though not so well as at 20°. Green color 
is not formed at 35°. 
Colony on Gelatine.—A small, opaque colony which rapidly liquefies into a 
large, greenish, slightly granular colony.. When liquefying, at first the granu- 
lar mass is associated at the center in a nucleus, but subsequently diffused 
throughout the liquid. 
Gelatine Stab.—Growth is slow. A shallow pit is formed, which becomes a 
deep, broad funnel. Later there is formed a liquid layer with a scum and a pre= 
cipitate, and a clear, green liquid between. 
Agar-Agar.— Thin, white, almost transparent, moist growth, spreading 
slightly. Agar becomes greenish. 
Potato.—Diffusely spreading, forming a thin, moist, brownish layer. 
Milk.—Curdles at 20° and also at 35°. Reaction is alkaline, casein becomes 
digested slowly. 
Bouilion.—A uniformly cloudy liquid with a slight scum. An abundant sedi- 
ment forms in six days. Liquid very cloudy toward the surface and slightly 
green. Later a very tenacious scum forms on the surface. 
No. 22. 
Locality,—Ellington creamery, November, 1892. 
Morphology.—A short, oval rod about 1.2 y.—2y. by I y. Variable in size. 
No spores. Stain uniform. 
Motility.—Stationary. 
Relation to Air.—Will not grow under mica. 
Temperature.—Very slight growth at 35°. 
Colony on Gelatine.—A rather thick, spreading colony formed with a central 
nucleus and a transparent edge, which shows radiant lines. 
Gelatine Stab.—Abundant needle growth. A thick, dirty white, opaque sur- 
face growth, which later sinks slightly in the gelatirie. 
Agar-Agar.—A dry, opaque layer with transparent edges. Spreads slightly. 
Potato.—-A white, narrow, raised streak, which later becomes yellowish. 
Milk.—No curdling. Reaction alkaline. Milk becomes slightly translucent. 
Bouillon.—Liquid uniformly cloudy, with no scum and only a slight precipi- 
tate. 

No. 23.: 
Locality.—Ellington creamery, November, 1892. 
Morphology.—A plump rod nearly as long as broad, 1.5 yp. by.8y. Some- 
times two will be seen, but no chains. Spores numerous. On potato it forms 
longer rods, 5 ys. in length. 
Motility.—Is motile. 
Relation to Air.—Grows under mica, producing gas bubbles. 
Temperature.—Grows well at 20° and 35°. 
Colony on Gelatine.—A small, round, clear, transparent colony, forming a 
white bead on the surface half a millimeter in diameter. In five days it reaches 
the diameter of one millimeter and is a small, round, glistening, white colony. 
Gelatine Stab.—A thin, rough, almost transparent surface growth. 
Agar-Agar.—A white, moderately thick, spreading layer. 
Potato.—An abundant white growth, glistening and moist, thick and heaped 
in mounds. Later distinctly brown. 
