29 “7 
fat Pe 
BACTERIA IN THE DAIRY, 55 
Motility,—Is motile. 
elation to Air.—Grows under mica. 
Temperature.—Grows well at 20° and 35°. 
Colony on Gelatine.—Clear, transparent colony under the surface, spreading 
on the surface into a clear, transparent, mounded colony with wrinkled edges. 
Gelatine Stab.—Abundant needle growth, beaded. Slight surface growth at 
first, later spreading into a thin, transparent surface layer. 
Agar-Agar.—White, raised growth, not characteristic. 
Potato.—Narrow, raised streak, with a yellowish tinge, spreading where 
moist. 
Milk.—Does not curdle. Is rendered alkaline and acquires a disagreeable 
odor and a bitter taste. After several weeks digested into a brownish liquid. 
Bouillon.—Cloudy with a scum and an abundant precipitate. 
. No. 31. 
Locality,—Water from a brook, January, 1893. 
Morphology.—A bacillus frequently forming long chains. Uniform stain and 
no spores. Dimensions 1.5 y. by .g p. 
Motility.— 
Relation to Air.—No growth under mica. 
Temperature.—No growth at 35°. 
Colony on Gelatine.—Small, round colony with radiating marks under the 
_ surface of the gelatine. On the surface a raised bead which becomes surrounded 
by a clear, granular pit. Granular central mass gradually breaking up and dis- 
persing in the pit. 
Gelatine Stab.—Liquefies slowly into a deep funnel, filled with green liquid. 
Agar-Agar.—White, smooth, moist, glistening growth, rather diffuse. Agar 
turned green. 
Potato.—Gray and dry, later becomes brownish. Quite thick. 
Milk.—Becomes slimy at 20°. Is strongly alkaline. Digestion of casein 
begins at once and milk rapidly becomes a yellowish green liquid completely 
digested. 
Boutlion.—Cloudy liquid with thick scum. Green one-half inch from top. 
A sediment collects. After ten days is green throughout and with very thick 
scum. 
With the above described organisms a large number of experi- 
ments in cream ripening have been performed, over 150 in all. 
About two-thirds of them are given in detail in the following 
table (12). The other experiments either agreed with those 
described and were for this reason unnecessary, or were omitted 
since they were isolated and too few to lead to reliable conclu- 
sions. ‘The table represents experiments extending from Novem- 
ber to April, which were all performed with organisms isolated 
from cream or water in the months of October or November. 
