78 STORRS AGRICULTURAL EXPERIMENT STATION. 
new milch cow, and the milk was of good flavor and consist- 
ency. Previous to the slaughter the cow had not been milked, 
a precaution taken to prevent the removal of any organisms 
that might chance to be in the milk ducts. ) 
The udder was taken from the cow immediately after slaugh- 
ter and brought to the laboratory, where it was examined and 
tested for bacteria. The examination was made within half 
an hour after the removal of the udder from the cow. All 
instruments used were sterilized. ‘whe skin of the udder was 
reflexed and a dorso-ventral incision made through the glandu- 
lar portion of the milk system and extending into the duct. 
From several portions of the udder inoculations were made 
with a sterile platinum needle directly into a variety of culture 
media, care being taken not to allow the milk of one portion 
of the udder to come in contact with the other portions. Inocu- 
lations were made in sterile milk, in serum tubes, upon agar 
slants, and in blue litmus-gelatin tubes. All culture tubes 
were then set aside, some at ordinary temperatures and others 
OE FOr | 
The results of inoculations were as follows: The milk tubes 
that had been placed at 37° C. curdled in 36 hours with an» 
acid reaction. Milk in tubes placed at 22° C. also developed an 
acid reaction, but did not curdle unless heated, and a little 
clear whey separated from it. The gelatin plates showed the 
presence of only a single species of bacteria, which was an acid 
producer and is described below. The other culture tubes 
placed at different temperatures showed, however, the presence 
of three species of bacteria, one of which was the same as that 
shown by the gelatin plates; the other two organisms had 
never been found in milk and were regarded as contamina- 
tions. ‘They were not acid organisms, and no especial atten- 
tion was paid to them. 
Cow No. 2.—In this case the autopsy showed a few mesen- 
‘ teric glands infected with tuberculosis and a single nodule in 
the liver. The lungs and udder appeared perfectly normal. 
The milk was good, and showed nothing that would indicate 
any abnormal condition of the udder. The udder was treated 
as in the previous case, but the inoculations were made ina 
_ different way. After reflexing the skin the various surfaces 
of the udder were burned with a hot knife, a stab was then 

