INVESTIGATIONS OF ACID ORGANISMS OF MILK. 79 
made by a long sterile needle through the flesh into various 
portions of the udder, and cultures were made as in the 
previous experiments. The udder was then opened by a 
dorso-ventral incision as before, and other cultures made. 
Inoculations were made as before into a variety of culture 
media, and these were kept at 22° C. and 37° C. for exam- 
ination. 
As before, the milk tubes at 37° C. curdled in 24-36 hours 
with an acid reaction, while those remaining at 22° did not 
curdle, though they became acid and curdled upon boiling. 
The plate cultures, as before, showed the presence of but one 
species of bacterium which produced perfectly characteristic 
colonies. The agar tubes and blood serum tubes in this exper- 
iment also showed the presence of this organism alone. In 
short, all of the inoculations made from this second animal 
agree in indicating the presence of one species of bacterium 
and one only. ‘The organism found was identical with the one 
found in the first cow and may, therefore, be regarded as a 
common udder bacterium in the cows of this particular herd. 
The organism in question is doubtless identical with one 
found many times by Conn in milk, and numbered by him in 
his Classification of Dairy Bacteria* as No. 60, and named 
M. acidi lactict I, ‘This organism he regards as belonging to 
a group to which belong also Nos. 113, 104, 78, and 58, these 
different varieties forming a group of coccus forms distinctively 
characteristic of milk. Apparently also the organism is the 
same as the micrococci found by Ward in his work on the 
udder and described by him. ‘The description which he gives 
is not quite identical with that given by Conn, but the differ- 
ences are so slight as to indicate that they are probably identt- 
cal organisms. 
The organism in question was tested in the laboratory by 
the ordinary culture means and found to have the following 
general characteristics: 
DESCRIPTION OF ORGANISM. FOUND IN TWO UDDERS. 
Morphology—Coccus usually found in pairs from .6 to Im in diameter. 
Motility—None. 
Temperature—Grows readily at ordinary temperature and rapidly at 37° C. 
Mica plate—Not determined. 

* See Report Storrs Station, 1899, pp. 13-68. 
