iy Z STORRS AGRICULTURAL EXPERIMENT STATION. 
SERIES III. ASEPTIC MILK. 
H. W. CONN AND W. A. STOCKING, JR. 
In the two series of experiments above reported one of the 
most surprising facts was the lack of any parallel observable 
between the numbers of bacteria present in the fresh milk and 
the numbers which were found at later periods—at 50 hours or 
at the time of curdling. Even though the samples of milk 
were kept at the same temperature and under conditions which 
were, so far as could be determined, identical, it was quite im- 
possible to reach any conclusion as to the number of bacteria 
which would be present at the end of 50 hours from the num- 

ber present at the outset. The numbers in the fresh milk in — 
the experiments already reported varied from 725 to 48,000 per 
cubic centimeter, and the number present in 50 hours varied 
from 32,000,000 to 1,800,000,000. The larger numbers in 
the later periods were not found in the milk that had larger 
numbers at the outset, but, as already pointed out, frequently 
just the contrary was true. This result, for which we could 
suggest no explanation, appeared to need further experiment- 
ing; and the question arose whether, if the number of bacteria 
could be reduced still lower, the same general results would be 
found. In other words, if milk could be secured which con- 
tained only a very small number of bacteria, would such milk 
contain at the end of 50 hours bacteria in numbers equal to 
those in milk obtained under less satisfactory conditions and 
containing at the outset more bacteria? Incidentally the expert- 
ments on this line would be of practical value in giving facts 
concerning the keeping property of aseptic milk. The series 
of experiments next undertaken, therefore, were designed for 
the purpose of comparing ordinary milk with milk obtained 
under exceptional precautions and kept with the same care. 
The method of experimenting was as follows. The milk 
from a single cow was used in all these experiments. One day 
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this milk was drawn into an ordinary open pail without extra’ 
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