78 STORRS AGRICULTURAL EXPERIMENT STATION. . te 
Colony on Gelatine.—A liquefying pit is formed with a nucleus and a cloudy 
edge. Differs from most liquefying colonies in not being clear round the rim, 
but uniformly cloudy to its extreme edge. 
Gelatine Stab Culture.—Growth is abundant Bloat the needle track. There is 
a slight surface growth which does not spread, is rather thick and does not 
very thoroughly liquefy the gelatine. | 
A gar-Agar.—A thick, moist layer with a sharp edge tinged with yellow. 
Potato.—No visible growth. 
Mitlk.—Is not affected either at 20° or at 35°. 
Bouillon.—Becomes slightly cloudy with a tough, yellowish sediment. After 
four weeks liquid becomes clear. 
No, 38. 
This is the well-known Bacillus subtilis. 
No. 40. 
Locality.—Found in a lot of milk from Uruguay, South America, at Chicago. 
Morphology.—A \arge micrococcus, size, .9 /. 
Motihty.—Non-motile. 
Relation to Air.—Will grow under mica plate. 
Colony on Gelatine.—¥orms a little pit with a center granular ye!lowish 
nucleus and an outer lobate granular ring. Sometimes the pit contains a 
yellowish granular mass without a rim. Sometimes there is formed an irregular 
lobate liquefying pit. 
Gelatine Stab Culture.—Slow growth. Forms a narrow funnel or sometimes 
a deep pit which is quite dry. There is formed a thick liquid with a yellowish 
sediment anda yellowish scum. ‘The gelatine never completely liquefies even 
after weeks of growth. 
A gar-Agar.—Forms a Naples yellow, rough, dry layer with a sharp edge. 
Potato.—Forms a Naples yellow, dry, mounded, thick layer, the color not so 
brilliant as on agar. 
Milk.—After five days at 35° the milk is curdled into a soft curd with no 
whey. The reaction is acid. After a month it becomes much harder with 
yellowish flakes and there is no digestion of the curd. ( 
Boutllon.—Remains clear with a slight, flaky scum and a slight sediment. 
After four weeks’ growth the whole liquid clears up retaining simply a slight 
yellowish sediment. 
No. 40 is closely allied to No. 2 described in the last Annual Report. It 
differs only in its ability to grow under mica and slightly in its growth in 
gelatine. 
No. 41. 
See page 57 of this Report. 
No. 43. 
Locality—From specimen of Uruguay milk. 
Morphology.—An oval micrococcus or a short rod not forming chains nor 
SDOrES. cuoize, Ott. DY (yz. 
Motilty.—Non-motile. 
Relation to Air.—Will grow under mica plate. 
Colony on Gelatine.—A smooth, round, slightly raised white bead. 

