80 STORRS AGRICULTURAL EXPERIMENT STATION. 
No. 46. 
Locality.—From Matzoon, at Jackson Park, Chicago. 
Morphology.—A short rod which forms chains of two or sometimes more. 
Size, .8 by .4 y on agar; slightly smaller on potato. 
Motility.— Possesses motion. 
Relation to Air.—Will grow under mica plate. 
Temperature.—Grows well at 35°, producing its color. Grows also at 20°. 
Colony on Gelatine.—A pit is formed with a rough crenate mass at the center. 
It spreads rapidly, becoming granular, and finally forms a pit with a nucleus 
and a clear rim. 
Gelatine Stab Culture.—An abundant needle growth is formed at first, and 
then a shallow pit at the surface, which slowly deepens, and after about eleven 
days forms a layer of liquefied gelatine half an inch deep. 
A gar-Agar.—A very thin, white, smooth layer which subsequently becomes 
yellowish. 
Potato.—A thick growth which is of a bright lemon yellow color. 
Milk.—The milk is very slowly affected. After about three weeks it is 
curdled into a soft mass which is either weakly alkaline or amphoteric in reac- 
tion. After six weeks’ growth it becomes slightly brown, and if kept at 35°, 
eventually is almost completely digested into a transparent brownish liquid 
which finally becomes almost mahogany colored. If the organism is cultivated 
at 35° for nine days and is then heated to a boiling temperature, the milk 
curdles. 
Boutllon.—Liquid becomes very cloudy, with a yellow precipitate forming. 
After four weeks it still remains cloudy with a dense precipitate. 
NO.147; 
Locality.—From the air at Middletown. 
Morphology.—A small micrococcus .4 y, in diameter. 
Motilty,—None. 
Relation to Aiy.—Does not grow under mica plate. 
Temperature.—Grows profusely at 35°. 
Colony on Gelatine.—A small, round, smooth, raised, snow-white bead. 
Gelatine Stab Cultuve.—An abundant growth along needle track with a thick, 
moist, but not widely spread surface growth. 
Agar-Agar.—A very moist, almost watery snow-white growth not very widely 
spreading. 
Potato.—Very watery, spreading over the surface of the potato. Snow 
white. 
Milk.—No effect upon the milk except a slight increase in alkalinity after 
about three weeks. At 35° there is sometimes a slight increase in thickness of 
the liquid. 
Boutllon.—A uniformly cloudy liquid rather clear at the surface. After four 
weeks the whole liquid clears up with a sediment. 
No. 48. 
Organism No, 48 is almost identical with Bacillus erythrogenes. It differs 
from the latter only in producing no growth on potato and in producing a 
brownish red rather than a blood-red milk, 



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