50 STORRS AGRICULTURAL EXPERIMENT STATION. 
light, at the proper angle, spectral colors will be observed. The 
stab growth in agar-agar is more abundant, and affords the only 
method of keeping it alive, because it soon dries up the surface 
and dies. 
CULTURE ON POTATO. 
Grows on potato very sparingly, in a thin, pearly, white 
layer, sometimes scarcely visible. 
BOUILLON. 
In milk-sugar bouillon the liquid becomes densely turbid. 
In a week it gradually settles, giving a light gray sediment in 
-a clear liquid. No scum or gas production is observed. Some- 
times a light-colored ring is formed on the glass at top of liquid. 
STERILIZED MILK. 
Cultures inoculated into sterile milk at 20° C. curdle it in 
‘twenty-four hours. At 35° C. it curdles milk in less than twelve 
hours. The curd is homogenous and of jelly consistency, so 
that the tube can be inverted without displacing the contents. 
The milk shrinks in curdling, leaving a concave surface on the 
top of the milk. After the curdling the milk undergoes no 
further visible change. A few drops of clear whey separates 
on top of the milk. The curd and clear liquid are intensely 
acid. No evidence of gas production or odor present: 
GENERAL REMARKS. 
It does not appear to produce spores. Hueppe describes 
‘it as a spore-forming species, but this appears to be an error. 
In staining specimens of these organisms the centers quite 
frequently remain unstained, which might lead to the miscon- 
ception that spores are present. The unstained portion does 
not show a glistening appearance, which is an optical effect 
produced by spores. 
The organism is quite difficult to cultivate. It lives but a 
short time on the surface of agar-agar, grows entirely below 
surface of gelatine, and very slowly in culture media at ordi- 
nary room temperatures. Its home seems to be milk, where. 
it flourishes to the best advantage at a temperature nearly that 
of the heat of the body. 
The habitat of this organism is a problem which is interest- 
ing for further investigation. A few experiments were tried 
to determine whether it came from hay dust or some other 
