L7a STORRS AGRICULTURAL EXPERIMENT STATION. 
of the experiment was kept apart, cooked by itself and served at a separate 
table. One of the gentlemen was at hand to make weighings and take samples 
of each food material used. The securing of satisfactory samples of most of 
the food materials, such as bread, potatoes, milk, sugar, etc., was by no means 
a difficult matter. With meats, however, accurate sampling was far from easy. 
In order to insure accuracy in the present instance the meats were treated in a 
special way. Each portion was carefully separated from the bone and finely 
chopped. This finely chopped material was set aside, carefully preserved and 
cooked. A portion, however, was taken to the laboratory for analysis. Especial 
effort was made by this and other means to make sure that the samples of the 
different food materials should represent as closely as possible the food as it was 
actually cooked and eaten. The separations of feces were made by the method 
above described. The urine of each day was collected, measured, and portions © 
were taken for the determination of the nitrogen by the Kjeldahl method. The 
further details are given in the descriptions which accompany the tabular state- 
ments of results. 
Lxperiment No, 6.—Kind of food: Mixed diet. Subject: Three chemists, 
aged 23, 26 and 28 years. Weight (without clothing): At the beginning, 61.7, 
60.3 and 68 kilos (136, 133 and 150 lbs.), and at the end, 63.5, 60.3 and 68 
kilos, respectively. The experiment began with breakfast, October roth, 1894, 
and ended with supper, October rgth, making 30 meals for each man. 
With the exceptions noted below all the food materials were analyzed and 
their heats of combustion were determined by the bomb calorimeter. The fuel 
values of the milk and cream were not determined, but were calculated from the 
percentage composition by the use of the factors 5.5, 9.3 and 4.1 for the fuel 
values of one gram each of protein, fats and carbohydrates respectively.> (ihe 
crackers and apples were not analyzed, but the composition was assumed from 
averages of the analyses of similar food materials as given in Bulletin No. 28 
of the Office of Experiment Stations of the U. S. Department of Agriculture on 
the *‘ Composition of American Food Materials.” The fuel values of these last 
named food materials were calculated by the use of the factors just referred to 
as employed for milk and cream. With the exception of the determinations of 
fuel values of the milk and cream, and the analyses and determinations of the 
fuel values of the crackers and apples, all of the food materials were analyzed 
for the purposes of the experiment and the heats of Seperate were deter-_ 
mined by the bomb calorimeter. 
It should be added that the figures for protein given in the table for all the 
animal foods, except oysters, cheese, milk and cream, are as obtained by differ- 
ence. For the animal foods just mentioned and the vegetable foods the protein 
is obtained by multiplying the nitrogen by the factor 6.25. 
The weights and composition of the food materials and feces, and the 
proportions of food materials digested, are given in table 50. Table sr gives 
the amounts of urine for each day and its nitrogen content, together with the 
weight and nitrogen content of the dried feces for the whole experiment. Table 
52 shows the income and outgo of nitrogen for each day covered by the experi- 
ment, together with the estimated gain or loss of protein. The computations 
for these tables are made as explained in the corresponding tables in the 
accounts of the respiration experiments above. 

