451311
JMTRANSLATION 730 (T730)
MEDICAL ZOOLOGY DEPARTMENT
UNITED STATES NAVAL MEDICAL RESEARCH UNIT No. 3
CAIRO, EGYPT

TRANSLATION FROM RUSSIAN. SIDENKO, V. P., GREKOV, V. S.,
STEPANKOVSKAYA, L. D., POLYAKOV, E. M., SOLOMKO, R. M. &
VOLYANSKAYA, E. A. (1973)*. 2001 Birds of southern Ukraine -reservoir of
tickborne encephalitis. 4101 Sborn. Trud. Ekol. Virus., 1 :140-144.

In accordance with the plan of coordinated study of arbovirus infection focus
in south European USSR, in Black Sea regions of Ukrainian Republic, we made
investigations to clarify tickborne encephalitis (TBE) infection in wild birds,
participation of these birds in virus circulation, and formation of TBE natural
foci in the Black Sea region, Ukraine.

MATERIAL AND METHODS

The material was collected in 4 bird migration and nesting areas: Stensov
marshy-floodplain of the Danube River, Mologa locality of the Dniester River
estuary, Bolshoi Sokoliny and Bolshoi Bokaisky islands, and Crimean foothills.
Birds were shot and trapped (during banding period) throughout the entire year.

Bird sera were examined by the complement fixation (CF) test in Tokatsy
microtitrator- In some cases, the bird heart suspension in borate solution
(pH-7.0) was also examined by CF. TBE virus served for diagnostic tests.

Before virological tests, the brain, and parenchymal organs were removed
from birds and placed in penicillin vials on dry ice.

Internal organ fractions of a few birds were used for preparing a suspension.
These fractions were ground in a porcelain mortar adding 10-fold
Hank's solution pH-0.7-7.6 or buffered physiological solution with 5-10%
normal rabbit or calf serum and 100-200 streptomycin or penicillin units
per 1 ml. The suspension was centrifuged at 2,000-2,500 rpm for 7-10 min.
The supernate was used (0.02 ml) for infection of newborn white mice (NWM).
Infected mice were observed for 21 days.

* Mechnikov Institute of Virology and Epidemiology, Odessa; Regional Sanitary
Epidemiological Station, Odessa.