Original Comntun ication s. 
besides this, there was a compound of oil and resin combined 
with colouring matter, and a substance of a light brown 
colour, very bitter taste, friable and very regular appearance, 
supposed to be a compound of a peculiar bitter principle, 
mixed with tannin and other matters. This was dissolved in 
alcohol and formed a beautiful red coloured tincture, which 
reddened litmus paper. Lime was then added, boiled, filtered 
and evaporated; a substance resembling the etherial residue 
remained interspersed with small, shining acicular crystals of 
a bitter taste, which property 1 am disposed to believe they 
owed to the bitter extract with which they were associated. 
The bark used in the last experiment was submitted to the 
action of boiling ether, which on cooling, deposited a substance 
of the consistence of wax which it resembled in all its pro- 
perties. 
Two ounces of the bark coarsely powdered were introduced 
into §viii of alcohol and exposed to a temperature of from 
105° to 120° F. The alcohol was then decanted and a fresh 
portion added and treated as before. The liquors were then 
united, and a solution of sub acetate of lead added to separate 
the colouring matter: after the insoluble portion subsided the 
clear liquor was separated, a little sulphuric acid was then 
added to the solution to separate any excess of sub-acetate 
of lead. This was filtered, and the alcohol distilled off. 
There remained in the retort an oily like substance together 
with a principle of a dirty white colour, curdled appearance, 
resembling the residue of the etherial tincture. Ammonia was 
then added to the liquor to precipitate any principle remaining 
in solution. The residue was then treated with a little sul- 
phuric acid, water, and animal charcoal, (previously treated 
with muriatic acid,) which upon evaporation deposited an 
abundant crystalline mass of a flaky appearance, resembling 
at first sulphate of quinine, but on cooling assumed a feathery 
appearance with a sharp saline taste, soluble in hot and cold 
water, insoluble in alcohol and ether, soluble in nitric acid, 
and resembled sulphate of ammonia in all its properties. 
One pound of coarsely powdered bark was boiled for half an 
•hour in one gallon of water acidulated with 3iss sulphuric acid. 
