TESTING  FOR  QUININE  AND  QUINIDIN. 
25 
B.  A  magma  of  phosphates  and  adhering  alkaloid  still  re- 
mained above  the  urinous  substratum ;  this  was  also  removed 
by  a  pipette  and  transferred  to  a  porcelain  capsule ;  evaporated 
to  dryness  at  212°,  and  this  residue  exhausted  by  ether ;  the 
ethereal  solution  evaporated  to  dryness  by  a  warm  water  bath, 
as  before,  gave  .61  additional  alkaloid. 
Therefore  fL_|_  ^=1.4  grain  of  alkaloid  were  obtained  by  these 
two  operations  from  the  eight  fluid  ounces  of  urine. 
Now  to  determine  whether  it  was  quinine,  the  following  process 
was  followed: — 
Test-fluid. — A  mixture  of  three  drachms  of  pure  acetic  acid, 
with  only  one  fluid  drachm  of  rectified  spirit  of  wine,  to  which 
six  drops  of  diluted  sulphuric  acid  were  added. 
One  drop  of  this  test-fluid  placed  on  a  glass  slide,  and  the 
merest  atom  of  the  alkaloid  added ;  time  given  for  solution  to 
take  place ;  then  upon  the  tip  of  a  very  fine  glass  rod  an  ex- 
tremely minute  drop  of  the  alcoholic  solution  of  iodine  added ; 
the  first  effect  is  the  production  of  the  yellow  or  cinnamon-colo- 
red compound  of  iodine  and  quinine,  which  forms  as  a  small  cir- 
cular spot ;  the  alcohol  separates  in  little  drops,  which  by  a  sort 
of  repulsive  movement  drive  the  fluid  away ;  after  a  time  the 
acid  liquid  again  flows  over  the  spot,  and  the  polarizing  crystals 
of  sulphate  of  iodo-quinine  are  slowly  produced  in  beautiful 
rosettes.    This  succeeds  best  without  the  aid  of  heat. 
To  render  these  crystals  evident,  it  merely  remains  to  bring  the 
glass  slide  upon  the  field 
.  o  .i         .  In  Fig.  land  2  the  inferior  tourmaline  and  selenite 
01  the  microscrope  With  stage  are  employed.    The  arrows  show  the  plane 
the  selenite  stage  and  sin-  of  the  tourmaline, 
gle  tourmaline  beneath  it;  FlG- 1,_ Red  Stase- 
instantly  the  crystals  as- 
sume the  two  complimen- 
tary colors  of  the  stage ; 
red  and  green,  supposing 
that  the  pink  stage  is 
employed,  or  blue  and 
yellow  provided  the  blue 
selenite  is  made  use  of. 
All  those  crystals  at  right 
angles  to  the  plane  of 
the  tourmaline  producing 
that  tint  which  an  analy- 
zing plate  of  tourmaline 
Would  produce  when  atA.Red.    jg.  Light  Green.    C.  Brownish  Yellow. 
