Am.  Jour.  Pharni. 
July,  1889. 
Pepsin  in  Urine. 
365 
in  the  tannic  acid  precipitate.  (3)  Nitrogen  in  the  precipitate  pro- 
duced by  saturation  with  magnesium  sulphate  (casein  -j-  globulin). 
(4.)  Casein  nitrogen  estimated  in  the  precipitate  produced  by  adding 
acetic  acid  to  milk.  (Approximate.)  (5.)  Globulin  nitrogen  ;  two 
estimations  ;  maximal,  obtained  by  the  difference  of  (3)  and  (4)  •  min- 
imal, estimated  in  the  precipitate  produced  by  magnesium  sulphate 
after  separating  the  casein  by  saturating  with  sodium  chloride.  (6.) 
Albumin -nitrogen,  estimated  in  the  precipitate  produced  by  adding 
tannic  acid  to  the  nitrate  after  the  separation  of  the  proteids,  precipi- 
itated  by  magnesium  sulphate  saturation.  (7.)  Non-proteid  nitrogen  is 
the  difference  between  (1)  and  (2). 
The  following  table  gives  the  results  in  the  analysis  of  five  speci- 
mens of  colostrum  : 
CD 
(2) 
(3.) 
(4.) 
(5.) 
(6.) 
(7.) 
1  ■.. 
2  
T232 
2-506 
1-  025 
2-  269 
0-887 
2-02 
0-56 
o-ioo 
j  0-248 
I  1-46 
0.138 
}  0-25 
0-207 
0-237 
3   
2-566 
2-461 
2147 
0534 
/  1-604 
I  1-613 
}  0-314 
0-086 
4  
2-222 
2-086 
1-903 
0-718 
f  0-3 
I  1-185 
}  0-183 
0-076 
5  
no 
0-716 
0-55 
•f     0  081 
\  0.166 
}  - 
PEPSIN  IN  NORMAL  AND  PATHOLOGICAL  URINE.1 
By  E.  Stadelmann. 
A  proteolytic  ferment  acting  like  pepsin  in  an  acid  medium  has 
been  found  by  many  observers  in  urine.  Neumeister  (1888),  brought 
forward  a  proof  that  this  is  pepsin,  when  he  showed  that  in  Carnivora 
injection  of  primary  albumoses  into  the  circulation  caused  the  appear- 
ance of  deuteroalbumose  in  the  urine ;  and  deuteroalbumose  when  in- 
jected was  similarly  converted  into  true  peptone. 
In  the  present  research,  the  question  whether  the  ferment  is  pepsin 
or  another  ferment  something  like  pepsin  was  investigated  by  per- 
forming experiments  with  the  ferment  outside  the  body  on  fibrin  ;  and 
the  results  may  be  thus  summarized : 
(1.)  The  ferment  is  true  pepsin,  as  by  its  action  all  the  products  of 
1  Zeit.  Biol.,  xxv.,  208-231.   Keprinted  from  Jour.  Chem.  Soc,  April,  p.  430. 
