478  American  Pharmaceutical  Association.    { A^tSm^is^.' 
The  results  reported  by  Lloyd  and  Kebler  last  year,  on  fluid  extract  of  coca,  were  as  follows  : 
Lloyd.  Kebler. 
Gravimetric.      Volumetric.  Gravimetric.  Volumetric. 
Brazil  wood   0-48  0-34.  0-53  0*35 
Cochineal   0*57  0-29  0*55  0*40 
Haematoxylon  ....  0*53  0-27  0*54  0-38 
Lacmoid   o*6o  0*35  0*50  038 
While  all  of  their  other  results  agreed  quite  closely,  this  position  was  considered  unsatis- 
factory. 
On  assaying  the  second  fluid  extract,  November  7th,  the  results  were  as  follows  : 
Lloyd.  Kebler. 
Using  Alcohol.                  Excluding  Alcohol.  Using  Alcohol. 
Gravimetric.   Volumetric.  Gravimetric.   Volumetric.  Gravimetric.  Volumetric. 
Brazil  wood  .    0*406             0*328                o  36                0*323  0*369      o"4i6    0*277  0*289 
Cochineal  .  .  .  0*37               0*290*               0*412               0*298*  0*416      0*416    0*277  0*286 
Haematoxylon,  0*406             0*315                0*376               0*321  0*384      0*370    0*290  0*293 
Average  .  .  0*394  0*311  0*383  0*314         0*389      0*401    0*315     o  289 
The  average  of  Lloyd's  gravimetric  results  is  0*388  ;  of  Kebler's  gravimetric,  0*395  ;  differ- 
ence, 0*007  Per  cent.    The  difference  of  the  volumetric  results  is  o*ou  per  cent. 
ASSAY  OF  POWDERED  COCA  LEAF. 
Since  the  mode  of  procedure  is  contained  in  the  report  of  the  workers,  the  directions  will 
not  be  repeated  here.  Prof.  Lloyd  reported  his  work  as  follows  :  50  grammes  of  the  powdered 
coca,  not  previously  dried  to  constant  weight,  were  put  into  a  one-half  gallon  bottle  and 
covered  with  500  grammes  of  chloroform  ether  (1  to  3)  mixture  ;  after  five  minutes'  rotating, 
50  c.c.  of  10  per  cent,  ammonia  were  added,  and  the  bottle  shaken  for  two  hours,  almost  con- 
tinuously, occasionally  cooling  the  bottle  in  cold  water.  Then  50  grammes  more  of  10  per 
cent,  ammonia  were  added,  well  shaken,  and  finally,  seven  times  50  grammes  of  the  ethereal 
fluid  drawn  off.  Six  of  them  were  assayed,  with  results  as  follows  :  (Probably  it  was  case  F, 
where  some  difficulty  was  experienced  in  pouring  off  50  grammes.) 
Gravimetric.  Volumetric. 
Haematoxylon  A,  1*102  per  cent.  1000  per  cent. 
"  3  drops  B,  0*994   11     "  0*957    "  " 
Cochineal  C,  1*038   "     "  0*950   "  " 
j  ^  •     ^   .<     .<  fa-o*927    "  " 
3  drops  D,  f-096  {b_o.^g2    „  „ 
Brazil  wood,  50  drops  ,  F,  1*002    "     "  0*952   "  " 
"         "      10      "   F,  0*944    "     "  0*897    "  ** 
Average  1029  per  cent.  0  952  per  cent. 
In  case  D,  it  was  attempted  to  see  what  effect  the  exclusion  of  alcohol  would  have  on  the 
results.  The  visible  effect  is  that  it  is  difficult  to  dissolve  the  alkaloid  completely  from  the 
resinous  material.  After  volumetric  results  were  found  too  low  (see  a),  the  same  determin- 
ation was  continued  by  adding  an  equal  bulk  of  alcohol,  which  caused  an  increase  in  the 
results  (see  b). 
In  these  experiments  the  titrations  were  carried  out  in  the  same  beaker  in  which  the 
gravimetric  results  were  obtained.  The  varnish-like  residues  were  dissolved  in  5  c.c.  of 
alcohol,  2  c.c.  of  N/10  sulphuric  acid  and  10  c.c.  of  water  were  added,  then  the  indicator, 
and  the  excess  of  acid  titrated  back  with  N/100  potassium  hydroxide.  In  each  case  1  c.c. 
of  N/10  was  added  again  and  the  excess  titrated  back  with  centinormal  potassium  hydroxide. 
Haematoxylon  and  cochineal  gave  sharp  changes  of  tint;  also  brazil  wood,  but  the  latter 
indicator  requires  a  trained  eye  to  see  the  change  plainly.  However,  the  solution  may  have 
deteriorated. 
Mr.  Kebler  proceeded  exactly  as  did  Professor  Lloyd,  except  that  the  shaking  was  intermit- 
tent instead  of  almost  continuous,  shaking  about  every  fifteen  minutes  during  two  hours. 
His  results  were  as  follows  : 
•Change  of  color  indistinct  except  when  using  more  of  the  indicator  than  prescribed. 
