A™'ctober,Pi902!m'}     American  Pharmaceutical  Association.  505 
such  active  principles.  The  points  to  be  decided  in  our  work  are : 
(1)  are  the  active  principle  or  principles  obtained  as  the  product  of 
our  assay  physiologically  active  in  the  sense  of  constricting  the  arte- 
rioles? and  (2)  is  the  mother  liquor  of  the  fluid  extract  from  which 
these  were  extracted  devoid  of  such  physiological  activity  ? 
Experiment  I. — Black  rooster,  weight  5  pounds.  Before  the 
injection,  the  wattles  and  comb  were  red  and  warm.  5  c.c.  fluid 
extract  of  best  Spanish  ergot  were  injected  hypodermically  at  10 
A.M. 
At  10.35  A-M-  Comb  bluish  but  warm,  wattles  still  red  and  warm. 
At  1 1  a.m.    Comb  and  wattles  blue  and  cooler. 
At  11.40  a.m.    Comb  and  wattles  very  blue  and  much  cooler. 
At  3.30  p.m.    Comb  and  wattles  still  very  blue  and  cool. 
Rooster's  bill  open  and  rooster  looking  quite  sick. 
Conclusion:  Fluid  extract  of  ergot  quite  active. 
Experiment  II. — The  Cornutine  of  Keller  obtained  from  5  c.c.  of 
this  same  fluid  extract  was  dissolved  in  weak  acetic  acid  and  diluted 
so  as  to  correspond  in  amount  to  the  original  fluid  extract  used. 
Injected  5  c.c.  of  this  liquid  at  10.35  A-M-  hypodermically  into  the 
same  rooster. 
At  11.30  a.m.  Comb  blue  and  cool  all  over,  bill  wide  open,  wat- 
tles bluish  and  cool. 
At  12  m.    Comb  much  bluer  and  cool,  wattles  blue  and  cool. 
At  1.30  p.m.  Comb  deep  blue  and  cool,  wattles  very  blue  and 
cool. 
At  4.20  p.m.  Comb  and  wattles  still  both  very  blue  all  over  and 
very  cool. 
Conclusion :  The  effect  of  the  Cornutine  of  Keller  is  identical  with 
that  of  the  fluid  extract. 
Experiment  III. — Filtrate  obtained  after  removing  all  the  Cornu- 
tine by  Keller's  method  of  assay  was  neutralized,  made  up  to  10  c.c. 
and  injected  hypodermically  into  the  same  rooster  at  9  45  a.m. 
At  1048  a.m.  Slight  bluing  at  two  tips  of  comb,  wattles  still  red 
and  both  warm. 
At  1.40  p.m.    Comb  and  wattles  still  warm  and  both  quite  red. 
Conclusion  :  All  of  the  fluid  extract  except  the  Cornutine  of 
Keller  does  not  cause  vaso-constriction,  and  it  is  hence  not  active 
physiologically. 
Experiment  IV. — Gray  and  white  rooster,  weight  5  pounds. 
